Hebei key laboratory of Chinese medicine research on cardio-cerebrovascular disease and Department of Biochemistry and Molecular Biology, College of Basic Medicine, Hebei University of Chinese Medicine, Shijiazhuang, Hebei, PR China.
Department of Physiology, Hebei University of Chinese Medicine, Shijiazhuang, Hebei, PR China.
Biomed Pharmacother. 2019 Feb;110:733-741. doi: 10.1016/j.biopha.2018.12.029. Epub 2018 Dec 13.
Gentianella acuta (Michx.) Hulten (G. acuta) has been widely used in Mongolian medicines for the treatment of cardiovascular diseases in Ewenki and Oroqen, Inner Mongolia autonomous region, China. The aim of this study was to investigate the effects and related mechanism of G. acuta on isoproterenol (ISO)-induced oxidative stress, fibrosis, and myocardial damage in rats. Male Sprague Dawley rats were randomly divided into the normal control group, ISO induced group and ISO+G. acuta treatment group. Rats were administered with ISO subcutaneously (5 mg/kg/day) for 7 days, and were orally administered simultaneously with aqueous extracts of G. acuta for 21 days. This investigation showed G. acuta treatment ameliorated cardiac structural disorder, excessive collagenous fiber accumulation and cardiac malfunction. Compared with the ISO induced model group, G. acuta treatment increased superoxide dismutase (SOD) activities and glutathione (GSH) level, prevented the rise of malondialdehyde (MDA), and decreased hydroxyproline contents in the heart tissues. Moreover, G. acuta reduced the expression of transforming growth factor β1 (TGF-β1) and connective tissue growth factor (CTGF), and inhibited the expression and activation of NF-κB-P65 in myocardial tissues. These results suggested that G. acuta protects against ISO-induced cardiac malfunction probably by preventing oxidative stress, and fibrosis, and the mechanism might be through inhibiting NF-κB pathway.
獐芽菜（Gentianella acuta (Michx.) Hulten）在中国内蒙古自治区的鄂温克族和鄂伦春族中被广泛用于治疗心血管疾病的蒙药。本研究旨在探讨獐芽菜对异丙肾上腺素（ISO）诱导的大鼠氧化应激、纤维化和心肌损伤的作用及其相关机制。雄性 Sprague Dawley 大鼠随机分为正常对照组、ISO 诱导组和 ISO+獐芽菜治疗组。大鼠皮下注射 ISO（5mg/kg/天）7 天，同时给予獐芽菜水提物 21 天。结果表明，獐芽菜治疗可改善心脏结构紊乱、胶原纤维过度积聚和心脏功能障碍。与 ISO 诱导模型组相比，獐芽菜治疗组可提高超氧化物歧化酶（SOD）活性和谷胱甘肽（GSH）水平，防止丙二醛（MDA）升高，并降低心脏组织中的羟脯氨酸含量。此外，獐芽菜可降低转化生长因子 β1（TGF-β1）和结缔组织生长因子（CTGF）的表达，抑制心肌组织中 NF-κB-P65 的表达和激活。这些结果表明，獐芽菜通过预防氧化应激和纤维化来保护 ISO 诱导的心脏功能障碍，其机制可能是通过抑制 NF-κB 通路。
