Yu Gaobo, Chen Fengqiong, Wang Yating, Chen Qiusen, Liu Hanlin, Tian Jin, Wang Mengxue, Ren Chunyuan, Zhao Qiang, Yang Fengjun, Sheng Yunyan, Wei Jinpeng, Zhang Yuxian
Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province 163319, China.
South China Agricultural University, Guangzhou, Guangdong Province 510642, China.
Food Chem X. 2022 Nov 15;16:100511. doi: 10.1016/j.fochx.2022.100511. eCollection 2022 Dec 30.
γ-aminobutyric acid (GABA) has been reported to improve stress resistance in plants. Nonetheless, little is known about the effects of GABA on the nutritional quality and regulatory mechanisms of edamame. Therefore, we analyzed the flavonoid and amino acid (AA) metabolism and the effects of GABA on the nutrient content of edamame seeds through physiological and metabolomic analyses. Exogenous GABA increased endogenous GABA metabolism and GABA transaminase activity and enhanced the oxoglutarate content, which entered into nitrogen metabolism and increased the activity and expression of nitrogen metabolism-related enzymes, to accumulate AAs and bioactive peptides. Meanwhile, exogenous GABA induced the metabolism of flavonoids, including total flavonoids, anthocyanins, 6''-o-malonyglycitin, glycitin, ononin, cyanin, and ginkgetin, by increasing the activity and expression of flavonoid biosynthetic enzymes. This is the first study to reveal that GABA effectively improves the nutritional quality of edamame through the accumulation of AAs, bioactive peptides, isoflavones, anthocyanins, sugars, and organic acids.
据报道，γ-氨基丁酸（GABA）可提高植物的抗逆性。然而，关于GABA对毛豆营养品质及调控机制的影响却知之甚少。因此，我们通过生理和代谢组学分析，研究了GABA对毛豆种子类黄酮和氨基酸（AA）代谢以及营养成分的影响。外源GABA增加了内源性GABA代谢和GABA转氨酶活性，提高了草酰戊二酸含量，其进入氮代谢并增加了氮代谢相关酶的活性和表达，从而积累了AA和生物活性肽。同时，外源GABA通过增加类黄酮生物合成酶的活性和表达，诱导了包括总黄酮、花青素、6''-O-丙二酰大豆黄素、大豆黄素、芒柄花苷、花青素和银杏黄素在内的类黄酮代谢。这是首次揭示GABA通过积累AA、生物活性肽、异黄酮、花青素、糖类和有机酸有效改善毛豆营养品质的研究。
