College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Instrumental Analysis Center of Qingdao University, Institute of Biomedical Engineering, Qingdao University, Qingdao, Shandong 266071, China.
College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Instrumental Analysis Center of Qingdao University, Institute of Biomedical Engineering, Qingdao University, Qingdao, Shandong 266071, China.
Ultrason Sonochem. 2023 Jan;92:106264. doi: 10.1016/j.ultsonch.2022.106264. Epub 2022 Dec 12.
In the luminol-O ECL system, O as an endogenous coreactant has the advantages of non-toxicity and stability. Improving the efficiency to generate radicals of O is a challenge currently. In this work, a strategy combining physical method - ultrasound and nanomaterial with unique physicochemical properties was designed to enhance the ECL signal of luminol-O system. Specifically, high-intensity focused ultrasound (HIFU) pretreatment as a non-invasive method could generate ROS (HO, O, OH•, O) in situ, triggering and boosting the ECL signal of luminol. In addition, 1T/2H MoS with excellent catalytic activity could catalyze the HO produced in situ, accelerate the oxidation of luminol and further enhance the ECL response. At the same time, combined with the catalytic hairpin assembly (CHA) reaction, the constructed ECL biosensing platform showed excellent performance for the detection of miRNA-155. The concentration range of 0.1 fM ∼ 1 nM with the detection limit as low as 0.057 fM were obtained. Furthermore, the ECL biosensor was also successfully applied to the determination of miRNA-155 in human serum samples. The established ECL sensing platform opens up a promising method for the detection of clinical biomarkers.
在鲁米诺-O ECL 体系中，O 作为内源性共反应物具有无毒、稳定的优点。提高 O 产生自由基的效率是目前面临的挑战。在这项工作中，设计了一种将物理方法-超声与具有独特物理化学性质的纳米材料相结合的策略，以增强鲁米诺-O 体系的 ECL 信号。具体而言，高强度聚焦超声（HIFU）预处理作为一种非侵入性方法，可以原位产生 ROS（HO、O、OH•、O），触发并增强鲁米诺的 ECL 信号。此外，具有优异催化活性的 1T/2H-MoS 可以催化原位产生的 HO，加速鲁米诺的氧化，进一步增强 ECL 响应。同时，结合催化发夹组装（CHA）反应，构建的 ECL 生物传感平台在检测 miRNA-155 方面表现出优异的性能。获得了 0.1 fM∼1 nM 的浓度范围和低至 0.057 fM 的检测限。此外，该 ECL 生物传感器还成功应用于人血清样品中 miRNA-155 的测定。所建立的 ECL 传感平台为临床生物标志物的检测开辟了一种很有前途的方法。
