通过用2-亚氨基硫杂环戊烷（4-巯基丁酸甲酯）对蛋白质进行修饰，向大肠杆菌核糖体添加巯基基团。 - Suppr

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超能文献

Addition of sulfhydryl groups to Escherichia coli ribosomes by protein modification with 2-iminothiolane (methyl 4-mercaptobutyrimidate).

作者信息

Jue R, Lambert J M, Pierce L R, Traut R R

出版信息

Biochemistry. 1978 Dec 12;17(25):5399-406. doi: 10.1021/bi00618a013.

DOI:10.1021/bi00618a013

PMID:365229

Abstract

摘要

相似文献

Addition of sulfhydryl groups to Escherichia coli ribosomes by protein modification with 2-iminothiolane (methyl 4-mercaptobutyrimidate).通过用2-亚氨基硫杂环戊烷（4-巯基丁酸甲酯）对蛋白质进行修饰，向大肠杆菌核糖体添加巯基基团。

Biochemistry. 1978 Dec 12;17(25):5399-406. doi: 10.1021/bi00618a013.

Disulfide cross-linking of Escherichia coli ribosomal proteins with 2-iminothiolane (methyl 4-mercaptobutyrimidate): evidence that the cross-linked protein pairs are formed in the intact ribosomal subunit.用2-亚氨基硫杂环戊烷（4-巯基丁酸甲酯）对大肠杆菌核糖体蛋白进行二硫键交联：交联蛋白对在完整核糖体亚基中形成的证据。

Biochemistry. 1978 Dec 12;17(25):5406-16. doi: 10.1021/bi00618a014.

Topography of ribosomal proteins of the Escherichia coli 30S subunit as studied with the reversible cross-linking reagent methyl 4-mercaptobutyrimidate.使用可逆交联试剂4-巯基丁酸甲酯研究大肠杆菌30S亚基核糖体蛋白的拓扑结构。

Biochemistry. 1974 May 21;13(11):2334-40. doi: 10.1021/bi00708a015.

Cross-linking of ribosomes using 2-iminothiolane (methyl 4-mercaptobutyrimidate) and identification of cross-linked proteins by diagonal polyacrylamide/sodium dodecyl sulfate gel electrophoresis.使用2-亚氨基硫杂环戊烷（4-巯基丁酸甲酯）对核糖体进行交联，并通过对角线聚丙烯酰胺/十二烷基硫酸钠凝胶电泳鉴定交联蛋白。

Methods Enzymol. 1979;59:534-50. doi: 10.1016/0076-6879(79)59112-5.

Identification by diagonal gel electrophoresis of nine neighboring protein pairs in the Escherichia coli 30 S ribosome crosslinked with methyl-4-mercaptobutyrimidate.通过对角线凝胶电泳鉴定与甲基-4-巯基丁酸亚胺交联的大肠杆菌30S核糖体中的九对相邻蛋白质对。

J Mol Biol. 1975 Oct 5;97(4):471-81. doi: 10.1016/s0022-2836(75)80054-4.

Methyl 4-mercaptobutyrimidate as a cleavable cross-linking reagent and its application to the Escherichia coli 30S ribosome.4-巯基丁酸甲酯作为一种可裂解交联剂及其在大肠杆菌30S核糖体中的应用。

Biochemistry. 1973 Aug 14;12(17):3266-73. doi: 10.1021/bi00741a019.

Identification of fifteen neighboring protein pairs in the Escherichia coli 50 S ribosomal subunit crosslinked with 2-iminothiolane.鉴定与2-亚氨基硫杂环戊烷交联的大肠杆菌50 S核糖体亚基中的十五对相邻蛋白质对。

J Mol Biol. 1979 Jan 25;127(3):243-63. doi: 10.1016/0022-2836(79)90328-0.

Identification of proteins at the binding site for protein S1 in 70 S ribosomes and 30 S subunits by cross-linking with 2-iminothiolane.通过与2-亚氨基硫杂环戊烷交联鉴定70S核糖体和30S亚基中蛋白质S1结合位点的蛋白质

J Biol Chem. 1981 Aug 10;256(15):8222-7.

Effect of a bifunctional imidoester on dissociation of 70S ribosomes in Escherichia coli.

Biochem Biophys Res Commun. 1973 May 1;52(1):35-42. doi: 10.1016/0006-291x(73)90950-9.

Identification of neighboring protein pairs in the Escherichia coli 30 S ribosomal subunit by crosslinking with methyl-4-mercaptobutyrimidate.通过与甲基-4-巯基丁酸亚胺交联鉴定大肠杆菌30S核糖体亚基中的相邻蛋白质对。

J Mol Biol. 1976 Oct 5;106(4):995-1015. doi: 10.1016/0022-2836(76)90348-x.

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