用2-亚氨基硫杂环戊烷（4-巯基丁酸甲酯）对大肠杆菌核糖体蛋白进行二硫键交联：交联蛋白对在完整核糖体亚基中形成的证据。 - Suppr

Suppr

超能文献

Disulfide cross-linking of Escherichia coli ribosomal proteins with 2-iminothiolane (methyl 4-mercaptobutyrimidate): evidence that the cross-linked protein pairs are formed in the intact ribosomal subunit.

作者信息

Lambert J M, Jue R, Traut R R

出版信息

Biochemistry. 1978 Dec 12;17(25):5406-16. doi: 10.1021/bi00618a014.

DOI:10.1021/bi00618a014

PMID:365230

Abstract

摘要

相似文献

Disulfide cross-linking of Escherichia coli ribosomal proteins with 2-iminothiolane (methyl 4-mercaptobutyrimidate): evidence that the cross-linked protein pairs are formed in the intact ribosomal subunit.用2-亚氨基硫杂环戊烷（4-巯基丁酸甲酯）对大肠杆菌核糖体蛋白进行二硫键交联：交联蛋白对在完整核糖体亚基中形成的证据。

Biochemistry. 1978 Dec 12;17(25):5406-16. doi: 10.1021/bi00618a014.

Addition of sulfhydryl groups to Escherichia coli ribosomes by protein modification with 2-iminothiolane (methyl 4-mercaptobutyrimidate).通过用2-亚氨基硫杂环戊烷（4-巯基丁酸甲酯）对蛋白质进行修饰，向大肠杆菌核糖体添加巯基基团。

Biochemistry. 1978 Dec 12;17(25):5399-406. doi: 10.1021/bi00618a013.

Cross-linking of ribosomes using 2-iminothiolane (methyl 4-mercaptobutyrimidate) and identification of cross-linked proteins by diagonal polyacrylamide/sodium dodecyl sulfate gel electrophoresis.使用2-亚氨基硫杂环戊烷（4-巯基丁酸甲酯）对核糖体进行交联，并通过对角线聚丙烯酰胺/十二烷基硫酸钠凝胶电泳鉴定交联蛋白。

Methods Enzymol. 1979;59:534-50. doi: 10.1016/0076-6879(79)59112-5.

Topography of ribosomal proteins of the Escherichia coli 30S subunit as studied with the reversible cross-linking reagent methyl 4-mercaptobutyrimidate.使用可逆交联试剂4-巯基丁酸甲酯研究大肠杆菌30S亚基核糖体蛋白的拓扑结构。

Biochemistry. 1974 May 21;13(11):2334-40. doi: 10.1021/bi00708a015.

Identification by diagonal gel electrophoresis of nine neighboring protein pairs in the Escherichia coli 30 S ribosome crosslinked with methyl-4-mercaptobutyrimidate.通过对角线凝胶电泳鉴定与甲基-4-巯基丁酸亚胺交联的大肠杆菌30S核糖体中的九对相邻蛋白质对。

J Mol Biol. 1975 Oct 5;97(4):471-81. doi: 10.1016/s0022-2836(75)80054-4.

Identification of fifteen neighboring protein pairs in the Escherichia coli 50 S ribosomal subunit crosslinked with 2-iminothiolane.鉴定与2-亚氨基硫杂环戊烷交联的大肠杆菌50 S核糖体亚基中的十五对相邻蛋白质对。

J Mol Biol. 1979 Jan 25;127(3):243-63. doi: 10.1016/0022-2836(79)90328-0.

Cross-linking studies on the 50 S ribosomal subunit of Escherichia coli with methyl 4-mercaptobutyrimidate.用4-巯基丁酸甲酯对大肠杆菌50 S核糖体亚基进行交联研究。

J Biol Chem. 1975 Dec 25;250(24):9434-6.

Identification of proteins at the binding site for protein S1 in 70 S ribosomes and 30 S subunits by cross-linking with 2-iminothiolane.通过与2-亚氨基硫杂环戊烷交联鉴定70S核糖体和30S亚基中蛋白质S1结合位点的蛋白质

J Biol Chem. 1981 Aug 10;256(15):8222-7.

Methyl 4-mercaptobutyrimidate as a cleavable cross-linking reagent and its application to the Escherichia coli 30S ribosome.4-巯基丁酸甲酯作为一种可裂解交联剂及其在大肠杆菌30S核糖体中的应用。

Biochemistry. 1973 Aug 14;12(17):3266-73. doi: 10.1021/bi00741a019.

Identification of neighboring protein pairs in the Escherichia coli 30 S ribosomal subunit by crosslinking with methyl-4-mercaptobutyrimidate.通过与甲基-4-巯基丁酸亚胺交联鉴定大肠杆菌30S核糖体亚基中的相邻蛋白质对。

J Mol Biol. 1976 Oct 5;106(4):995-1015. doi: 10.1016/0022-2836(76)90348-x.

引用本文的文献

A simple bioconjugate attachment protocol for use in single molecule force spectroscopy experiments based on mixed self-assembled monolayers.一种基于混合自组装单分子层用于单分子力谱实验的简单生物共轭连接方案。

Int J Mol Sci. 2012 Oct 19;13(10):13521-41. doi: 10.3390/ijms131013521.

Topography of the C. coli 5S RNA-protein complex as determined by crosslinking with dimethyl suberimidate and dimethyl-3,3'-dithiobispropionimidate.通过与辛二酸二甲酯亚胺和二甲基-3,3'-二硫代双丙酰亚胺交联确定的大肠弯曲杆菌5S RNA-蛋白质复合物的拓扑结构。

Nucleic Acids Res. 1981 Feb 25;9(4):993-1004. doi: 10.1093/nar/9.4.993.

Topography and stoichiometry of acidic proteins in large ribosomal subunits from Artemia salina as determined by crosslinking.通过交联确定卤虫大核糖体亚基中酸性蛋白的拓扑结构和化学计量

Proc Natl Acad Sci U S A. 1987 Aug;84(16):5580-4. doi: 10.1073/pnas.84.16.5580.

In vivo administration of lymphocyte-specific monoclonal antibodies in nonhuman primates. In vivo stability of disulfide-linked immunotoxin conjugates.在非人类灵长类动物体内给予淋巴细胞特异性单克隆抗体。二硫键连接的免疫毒素缀合物的体内稳定性。

J Clin Invest. 1986 Mar;77(3):977-84. doi: 10.1172/JCI112399.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。

立即体验