Personality Psychology and Psychological Assessment, University of Leipzig, Germany; Center for Adaptive Rationality, Max Planck Institute for Human Development, Germany.
University of New South Wales, Sydney, Australia.
Psychoneuroendocrinology. 2023 Mar;149:105994. doi: 10.1016/j.psyneuen.2022.105994. Epub 2022 Dec 5.
Salivary steroid immunoassays are widely used in psychoneuroendocrinological studies of menstrual cycle phase, puberty, and menopause. Though manufacturers advertise their assays as suitable, they have not been rigorously validated for these purposes. We collated data from eight menstrual cycle studies across > 1200 female participants and > 9500 time points. Seven studies collected saliva and one collected serum. All assayed estradiol and progesterone and had an independent measure of cycle phase (LH-surge, menstrual onset). In serum, cycle phase measures strongly predicted steroid concentrations. In saliva, cycle phase poorly predicted estradiol values, which showed an upward bias compared to expectations from serum. For salivary progesterone, predictability from cycle phase was mixed, low for enzyme-linked assays and moderate for tandem mass spectrometry. Imputing the population-average serum steroid changes from cycle phase may yield more valid values of hormonal changes for an independent person than directly assessing their hormone levels using salivary immunoassays.
唾液类固醇免疫分析广泛应用于月经周期、青春期和更年期的神经精神内分泌研究。尽管制造商宣传他们的检测方法适用于这些目的，但这些检测方法尚未经过严格验证。我们汇集了来自 8 项月经周期研究的数据，涉及超过 1200 名女性参与者和超过 9500 个时间点。其中 7 项研究采集了唾液，1 项研究采集了血清。所有检测都测定了雌二醇和孕酮，并对周期阶段（LH 激增、月经开始）有独立的测量。在血清中，周期阶段的测量值可以很好地预测类固醇浓度。在唾液中，周期阶段对雌二醇值的预测能力较差，与血清中的预期值相比，雌二醇值存在向上的偏差。对于唾液中的孕激素，来自周期阶段的可预测性各不相同，酶联免疫测定法的预测性较低，串联质谱法的预测性适中。从周期阶段推断人群平均血清类固醇变化可能比直接使用唾液免疫分析评估个体的激素水平更能得到激素变化的更有效值。
