Stazione Leonel, Sambucetti Pablo D, Norry Fabian M
Instituto de Ecología, Genética y Evolución de Buenos Aires (IEGEBA) - CONICET, Universidad de Buenos Aires, C-1428-EHA Buenos Aires, Argentina; Facultad de Ciencias Exactas y Naturales, Departamento de Ecología, Genética y Evolución, Universidad de Buenos Aires, C-1428-EHA Buenos Aires, Argentina.
Instituto de Ecología, Genética y Evolución de Buenos Aires (IEGEBA) - CONICET, Universidad de Buenos Aires, C-1428-EHA Buenos Aires, Argentina; Facultad de Ciencias Exactas y Naturales, Departamento de Ecología, Genética y Evolución, Universidad de Buenos Aires, C-1428-EHA Buenos Aires, Argentina.
J Insect Physiol. 2023 Jan;144:104468. doi: 10.1016/j.jinsphys.2022.104468. Epub 2022 Dec 14.
In insects, mating ability at elevated temperature can be relevant for adaptation to heat-stressed environments and global warming. Here, we examined copulation latency (T1), copulation duration (T2), and mating frequency (T3, an index of mating success) in two related sets of recombinant inbred lines (RIL) in Drosophila melanogaster at both elevated (33 °C) and benign (25 °C) temperatures. One of these RIL sets (RIL-SH2) was shown to be consistently more resistant in both heat knockdown and heat-shock survival assays than its related set (RIL-D48) in previous studies. Negative correlations across RILs were found between T1 and T3 in this study. Flies from the heat-resistant set of RIL (RIL-SH2) were better able to mate at elevated temperature than flies from the heat-susceptible set (RIL-D48). Quantitative trait locus (QTL) mapping identified temperature-dependent QTLs for all traits (T1, T2 and T3) on all the three major chromosomes. Mating success at elevated temperature was found to be influenced by multiple QTLs. At elevated temperature, several QTLs for mating traits co-localized with QTLs that were previously associated with thermotolerance. The genetic basis for T1, T2 and T3 at the elevated temperature was found to be largely different from the genetic basis controlling the variation for mating success at benign temperature, as there was only a very low (or even null) number of QTLs overlapping across temperatures.
在昆虫中，高温下的交配能力可能与适应热应激环境和全球变暖有关。在此，我们研究了黑腹果蝇两组相关重组自交系（RIL）在高温（33°C）和适宜温度（25°C）下的交配潜伏期（T1）、交配持续时间（T2）和交配频率（T3，交配成功的指标）。在之前的研究中，其中一组RIL（RIL-SH2）在热击倒和热休克存活试验中始终比其相关组（RIL-D48）更具抗性。本研究发现，RIL之间T1和T3呈负相关。来自耐热RIL组（RIL-SH2）的果蝇在高温下比来自热敏感组（RIL-D48）的果蝇更能成功交配。数量性状基因座（QTL）定位在所有三条主要染色体上鉴定出了所有性状（T1、T2和T3）的温度依赖性QTL。发现高温下的交配成功受多个QTL影响。在高温下，几个交配性状的QTL与先前与耐热性相关的QTL共定位。发现高温下T1、T2和T3的遗传基础与控制适宜温度下交配成功变异的遗传基础有很大不同，因为不同温度下重叠的QTL数量非常少（甚至没有）。
