Stazione L, Norry F M, Sambucetti P
Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Ecología, Genética y Evolución, Buenos Aires, Argentina; CONICET, Universidad de Buenos Aires, Instituto de Ecología, Genética y Evolución (IEGEBA), Buenos Aires, Argentina.
Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Ecología, Genética y Evolución, Buenos Aires, Argentina; CONICET, Universidad de Buenos Aires, Instituto de Ecología, Genética y Evolución (IEGEBA), Buenos Aires, Argentina.
J Therm Biol. 2019 Feb;80:172-177. doi: 10.1016/j.jtherbio.2019.02.001. Epub 2019 Feb 2.
Reproduction is strongly influenced by environmental temperature in insects. At high temperature, mating success could be influenced not only by basal (non-inducible) thermotolerance but also by inducible plastic responses. Here, mating success at high temperature was tested in flies carrying contrasting genotypes of heat resistance in Drosophila melanogaster. The possible heat-hardening effect was tested. Mating success did not differ between heat-resistant and heat-sensitive genotypes when tested both at high (33 °C) and benign (25 °C) temperature, independently of the heat-hardening status. Importantly, heat-hardening pre-treatment increased in a 70% the number of matings at 33 °C in a mass-mating experiment. Further, mating latency at 33 °C was shorter with heat hardening than without it in single-pair assays Heat-hardening had previously been showed to improve short-term thermotolerance in many organisms including Drosophila, and the present results show that heat hardening also improve mating success at elevated temperature. Previous exposures to a mild heat stress improve short-term mating success as a plastic response of ecological relevance. Such heat-hardening effects on mating success should be relevant for predicting potential evolutionary responses to any possible current scenery of global warming, as well as in sterile insect release programs for pest control in elevated temperature environments.
昆虫的繁殖受到环境温度的强烈影响。在高温下，交配成功率不仅可能受到基础（非诱导性）耐热性的影响，还可能受到诱导性可塑性反应的影响。在此，对携带黑腹果蝇耐热性不同基因型的果蝇在高温下的交配成功率进行了测试。对可能的热硬化效应进行了测试。在高温（33°C）和适宜温度（25°C）下进行测试时，耐热型和热敏型基因型之间的交配成功率没有差异，且与热硬化状态无关。重要的是，在大规模交配实验中，热硬化预处理使33°C时的交配次数增加了70%。此外，在单对试验中，33°C时经过热硬化处理的交配潜伏期比未经过热硬化处理的要短。此前已表明，热硬化能提高包括果蝇在内的许多生物体的短期耐热性，目前的结果表明，热硬化还能提高高温下的交配成功率。先前暴露于轻度热应激可作为一种具有生态相关性的可塑性反应，提高短期交配成功率。这种热硬化对交配成功率的影响对于预测对当前全球变暖任何可能情景的潜在进化反应，以及在高温环境下害虫防治的不育昆虫释放计划中都应具有重要意义。
