Stern C D, Keynes R J
Department of Human Anatomy, Oxford, UK.
Development. 1987 Feb;99(2):261-72. doi: 10.1242/dev.99.2.261.
We have investigated the interactions between the cells of the rostral and caudal halves of the chick somite by carrying out grafting experiments. The rostral half-sclerotome was identified by its ability to support axon outgrowth and neural crest cell migration, and the caudal half by the binding of peanut agglutinin and the absence of motor axons and neural crest cells. Using the chick-quail chimaera technique we also studied the fate of each half-somite. It was found that when half-somites are placed adjacent to one another, their interactions obey a precise rule: sclerotome cells from like halves mix with each other, while those from unlike halves do not; when cells from unlike halves are adjacent to one another, a border is formed. Grafting quail half-somites into chicks showed that the fates of the rostral and caudal sclerotome halves are similar: both give rise to bone and cartilage of the vertebral column, as well as to intervertebral connective tissue. We suggest that the rostrocaudal subdivision serves to maintain the segmental arrangement when the mesenchymal sclerotome dissociates, so that the nervous system, vasculature and possibly vertebrae are patterned correctly.
我们通过进行移植实验，研究了鸡胚体节头侧和尾侧两半部分细胞之间的相互作用。头侧半体节通过其支持轴突生长和神经嵴细胞迁移的能力来确定，尾侧半体节则通过花生凝集素的结合以及运动轴突和神经嵴细胞的缺失来确定。利用鸡 - 鹌鹑嵌合体技术，我们还研究了每个半体节的命运。结果发现，当半体节彼此相邻放置时，它们的相互作用遵循精确的规则：来自相似半体节的硬骨细胞相互混合，而来自不同半体节的则不会；当来自不同半体节的细胞彼此相邻时，会形成一个边界。将鹌鹑半体节移植到鸡胚中表明，头侧和尾侧硬骨半体节的命运相似：两者都产生脊柱的骨骼和软骨以及椎间结缔组织。我们认为，当头侧 - 尾侧细分在间充质硬骨细胞解离时有助于维持节段排列，从而使神经系统、脉管系统以及可能的椎骨能够正确地形成模式。
