Wang Hongyang, Wang Yuefeng, Kang Chuanzhi, Wang Sheng, Zhang Yan, Yang Guang, Zhou Li, Xiang Zengxu, Huang Luqi, Liu Dahui, Guo Lanping
State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.
Key Laboratory of Biology and Cultivation of Herb Medicine, Ministry of Agriculture and Rural Affairs, Beijing, China.
Front Plant Sci. 2022 Dec 2;13:1032480. doi: 10.3389/fpls.2022.1032480. eCollection 2022.
is an important medicinal plant in traditional Chinese medicine, its rhizome is rich of volatile secondary metabolites with medicinal values and is largely demanded in modern markets. Currently, supply of high-yield, high-quality is mainly achieved cultivation. Certain soil microbes can benefit plant growth, secondary metabolism and induce resistance to environmental stresses. Hence, studies on the effects of soil microbe communities and isolates microorganisms on is extremely meaningful for future application of microbes on cultivation. Here we investigated the effects of the inoculation with an entire soil microbial community on the growth, resistance to drought, and accumulation of major medicinal compounds (hinesol, β-eudesmol, atractylon and atractylodin) of . We analyzed the interaction between and the soil microbes at the phylum and genus levels under drought stress of different severities (inflicted by 0%, 10% and 25% PEG6000 treatments). Our results showed that inoculation with soil microbes promoted the growth, root biomass yield, medicinal compound accumulation, and rendered drought-resistant traits of , including relatively high root:shoot ratio and high root water content under drought. Moreover, our results suggested drought stress was more powerful than the selectivity of in shaping the root-associated microbial communities; also, the fungal communities had a stronger role than the bacterial communities in protecting from drought. Specific microbial clades that might have a role in protecting from drought stress were identified: at the genus level, the rhizospheric bacteria , and , and rhizospheric fungi , , and , the root endophytic bacteria , , and , and the root endophytic fungus were closely associated with under drought stress. Additionally, we acquired several endophytic , and strains and verified they had differential promoting effects on the medicinal compound accumulation in root. This study reports the interaction between and soil microbe communities under drought stress, and provides insights for improving the outcomes in farming applying microbe inoculation.
是传统中药中的一种重要药用植物，其根茎富含具有药用价值的挥发性次生代谢产物，在现代市场上需求量很大。目前，高产、优质的供应主要通过栽培实现。某些土壤微生物可以促进植物生长、次生代谢并诱导对环境胁迫的抗性。因此，研究土壤微生物群落和分离微生物对的影响对于微生物在栽培中的未来应用极具意义。在这里，我们研究了接种完整土壤微生物群落对的生长、抗旱性以及主要药用化合物（苍术醇、β-桉叶醇、苍术酮和苍术素）积累的影响。我们分析了在不同严重程度的干旱胁迫（由0%、10%和25% PEG6000处理造成）下，与土壤微生物在门和属水平上的相互作用。我们的结果表明，接种土壤微生物促进了的生长、根生物量产量、药用化合物积累，并赋予了抗旱特性，包括在干旱条件下相对较高的根冠比和高根含水量。此外，我们的结果表明干旱胁迫在塑造根际微生物群落方面比的选择性更强大；而且，真菌群落在保护免受干旱方面比细菌群落发挥了更强的作用。确定了可能在保护免受干旱胁迫中起作用的特定微生物类群：在属水平上，根际细菌、和，以及根际真菌、、和，根内生细菌、和，以及根内生真菌在干旱胁迫下与密切相关。此外，我们获得了几种内生、和菌株，并验证了它们对根中药用化合物积累具有不同的促进作用。本研究报告了干旱胁迫下与土壤微生物群落之间的相互作用，并为通过接种微生物改善种植结果提供了见解。
