Fatimah Siti, Syafii Mohammad, Zulaeha Siti, Haristianita Mega Dewi, Purwoko Devit
Agrotechnology Study Program, Faculty of Agriculture, Trunojoyo University, Unijoyo Campus, Telang, PO. BOX 2 Kamal - Bangkalan, East Java, Indonesia.
Research Center for Genetic Engineering, Research Organization for Life Sciences and Environment, National Research and Innovation Agency, Building No. 630 PUSPIPTEK South Tangerang Banten 15314, Indonesia.
Data Brief. 2022 Nov 17;45:108760. doi: 10.1016/j.dib.2022.108760. eCollection 2022 Dec.
The assembly of dihaploid rice plants through anther culture was constrained due to the recalcitrant properties. A comprehensive investigation of gene expression patterns among rice varieties with recalcitrant and non-recalcitrant anthers will help to understand the cellular mechanisms and biological processes of recalcitrant properties in rice anther cultures. Therefore, we performed RNA sequencing and analysis on the anthers of three selected Indonesian rice varieties with opposite recalcitrant properties. The varieties are Fatmawati with non-recalcitrant properties, IR64 recalcitrant and Tarabas unknown. The Illumina NextSeq PE150 sequencer was used to generate a total crude nucleotide of approximately 41.21 Gb in size. From 272,239,682 total paired final raw reads, 137,343,391 total net reads were obtained and uploaded to NCBI's Sequence Read Archive (SRA) repository under BioProject accession number PRJNA856048. This dataset allowed us to identify and profile all expressed genes with functions associated with recalcitrant and non-recalcitrant properties. In addition, the transcriptome data obtained will be valuable for the discovery of potential gene markers and functional SNPs associated with functional traits to assist rice breeding programs through the development of Marker Assisted Selection (MAS).
由于花药培养具有顽拗特性，通过花药培养组装双单倍体水稻植株受到限制。对具有顽拗花药和非顽拗花药的水稻品种之间的基因表达模式进行全面研究，将有助于了解水稻花药培养中顽拗特性的细胞机制和生物学过程。因此，我们对三个具有相反顽拗特性的印度尼西亚水稻品种的花药进行了RNA测序和分析。这些品种分别是具有非顽拗特性的法特玛瓦蒂、顽拗的IR64和未知的塔拉巴斯。使用Illumina NextSeq PE150测序仪生成了总大小约为41.21 Gb的原始核苷酸。从总共272,239,682对最终原始读段中，获得了137,343,391条总净读段，并上传至NCBI的序列读取存档（SRA）库，生物项目登录号为PRJNA856048。该数据集使我们能够识别和分析所有与顽拗和非顽拗特性相关的功能表达基因。此外，获得的转录组数据对于发现与功能性状相关的潜在基因标记和功能性单核苷酸多态性（SNP）具有重要价值，有助于通过标记辅助选择（MAS）的发展来辅助水稻育种计划。
