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Trimmomatic: a flexible trimmer for Illumina sequence data.Trimmomatic:一款适用于 Illumina 测序数据的灵活修剪工具。
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TopHat2: accurate alignment of transcriptomes in the presence of insertions, deletions and gene fusions.TopHat2:在存在插入、缺失和基因融合的情况下对转录组进行精确比对。
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Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks.RNA-seq 实验中使用 TopHat 和 Cufflinks 的差异基因和转录本表达分析。
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来自马来西亚有色和无色水稻品种全稻谷的RNA测序数据。

RNA-seq data from whole rice grains of pigmented and non-pigmented Malaysian rice varieties.

作者信息

Zainal-Abidin Rabiatul-Adawiah, Zainal Zamri, Mohamed-Hussein Zeti-Azura, Abu-Bakar Norliza, Ab Razak Mohd Shahril Firdaus, Simoh Sanimah, Sew Yun Shin

机构信息

Malaysian Agricultural Research & Development Institute (MARDI), 43300 Serdang, Selangor, Malaysia.

Institute of Systems Biology (INBIOSIS), Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia.

出版信息

Data Brief. 2020 Mar 16;30:105432. doi: 10.1016/j.dib.2020.105432. eCollection 2020 Jun.

DOI:10.1016/j.dib.2020.105432
PMID:32280737
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7138961/
Abstract

Pigmented rice is enriched with antioxidants, macro- and micronutrients. A comprehensive investigation of the gene expression patterns among the pigmented rice varieties would help to understand the cellular mechanism and biological processes of rice grain pigmentation. Hence, we performed RNA sequencing and analysis on the whole grain of dehusked mature seeds of selected six Malaysian rice varieties with varying grain pigmentations. These varieties were black rice (BALI and Pulut Hitam 9), red rice (MRM16 and MRQ100) and white rice (MR297 and MRQ76). Illumina HiSeq™ 4000 sequencer was used to generate total raw nucleotides of approximately 53 Gb in size. From 353,937,212 total paired-end raw reads, 340,131,496 total clean reads were obtained. The raw reads were deposited into European Nucleotide Archive (ENA) database and can be accessed via accession number PRJEB34340. This dataset allows us to identify and profile all expressed genes with functions related to nutritional traits (i.e. antioxidants, folate and amylose content) and quality trait (i.e. aroma) across both pigmented and non-pigmented rice varieties. In addition, the transcriptome data obtained will be valuable for discovery of potential gene markers and functional SNPs related to functional traits to assist in rice breeding programme.

摘要

有色米富含抗氧化剂、大量营养素和微量营养素。对有色米品种间基因表达模式进行全面研究,将有助于了解水稻籽粒色素沉着的细胞机制和生物学过程。因此,我们对所选的六个具有不同籽粒色素沉着的马来西亚水稻品种的脱壳成熟种子的全粒进行了RNA测序和分析。这些品种包括黑米(BALI和Pulut Hitam 9)、红米(MRM16和MRQ100)和白米(MR297和MRQ76)。使用Illumina HiSeq™ 4000测序仪生成了大小约为53 Gb的总原始核苷酸。从353,937,212条总双端原始读段中,获得了340,131,496条总清洁读段。原始读段已存入欧洲核苷酸档案库(ENA)数据库,可通过登录号PRJEB34340访问。该数据集使我们能够识别和分析所有与营养性状(即抗氧化剂、叶酸和直链淀粉含量)和品质性状(即香气)相关的功能基因,这些基因存在于有色和无色水稻品种中。此外,获得的转录组数据对于发现与功能性状相关的潜在基因标记和功能性单核苷酸多态性(SNP)具有重要价值,有助于水稻育种计划。