Centre for Medical Biotechnology, Maharshi Dayanand University, Rohtak, Haryana, India.
Department of Microbiology, Maharshi Dayanand University, Rohtak, Haryana, India.
Methods Mol Biol. 2023;2610:109-127. doi: 10.1007/978-1-0716-2895-9_10.
Influenza A virus H1N1, a respiratory virus transmitted via droplets and responsible for the global pandemic in 2009, belongs to the Orthomyxoviridae family, a single-negative-stranded RNA. It possesses glycoprotein spikes neuraminidase (NA), hemagglutinin (HA), and a matrix protein named M2. The Covid-19 pandemic affected the world population belongs to the respiratory virus category is currently mutating, this can also be observed in the case of H1N1 influenza A virus. Mutations in H1N1 can enhance the viral capacity which can lead to another pandemic. This virus affects children below 5 years, pregnant women, old age people, and immunocompromised individuals due to its high viral capacity. Its early detection is necessary for the patient's recovery time. In this book chapter, we mainly focus on the detection methods for H1N1, from traditional ones to the most advance including biosensors, RT-LAMP, multi-fluorescent PCR.
甲型 H1N1 流感病毒是一种通过飞沫传播的呼吸道病毒，是 2009 年全球大流行的罪魁祸首，属于正粘病毒科，为单负链 RNA 病毒。它拥有糖蛋白刺突神经氨酸酶（NA）、血凝素（HA）和一种名为 M2 的基质蛋白。目前正在发生变异的新冠病毒属于呼吸道病毒类别，也会对世界人口产生影响。甲型 H1N1 流感病毒的突变可以增强其病毒能力，这可能导致另一场大流行。由于其高病毒能力，该病毒会影响 5 岁以下儿童、孕妇、老年人和免疫功能低下者。早期检测对于患者的康复时间非常重要。在本章中，我们主要关注 H1N1 的检测方法，从传统方法到最先进的包括生物传感器、RT-LAMP 和多重荧光 PCR。
