Department of Laboratory Medicine, Shenzhen Baoan District Songgang People's Hospital, Shenzhen, China.
Department of Laboratory Medicine, Shenzhen Longhua District Central Hospital, Shenzhen, China.
Scand J Clin Lab Invest. 2023 Feb;83(1):18-22. doi: 10.1080/00365513.2022.2155990. Epub 2022 Dec 19.
Hb variants prevalent in China are different from those in other countries. We aimed to assess the interference from Hb variants found in China on HbA1c measurement. All Hb variants were confirmed using Sanger sequencing. HbA1c was measured using a capillary electrophoresis method (Capillarys 3 OCTA), two cation-exchange high-performance liquid chromatography methods (ADAMS HA-8180V and HLC-723 G8 standard mode), an immunoassay (Cobas c501), and a boronate affinity chromatography method (Premier Hb9210). Premier Hb9210 was used as a comparative method. A total of 16 species of Hb variants were identified in 102 variant carriers. The most common variant was Hb E, followed by Hb Q-Thailand, Hb New York and Hb J-Bangkok. Clinically significant interference was observed for the Capillarys 3 OCTA (two Hb variants), ADAMS HA-8180V (seven Hb variants), HLC-723 G8 (14 Hb variants), and Cobas c501 (two Hb variants). The proportion of unacceptable HbA1c results was 13.7% for Capillarys 3 OCTA, 52.9% for HA-8180V, 83.3% for HLC-723 G8, and 3.9% for Cobas c501. Hb variants in China severely affect the accuracy of some commonly used HbA1c methods.
在中国流行的血红蛋白变异与其他国家不同。我们旨在评估在中国发现的血红蛋白变异对 HbA1c 测量的干扰。所有的血红蛋白变异均通过 Sanger 测序确认。HbA1c 采用毛细管电泳法（Capillarys 3 OCTA）、两种阳离子交换高效液相色谱法（ADAMS HA-8180V 和 HLC-723 G8 标准模式）、免疫测定法（Cobas c501）和硼酸亲和层析法（Premier Hb9210）进行测量。Premier Hb9210 用作比较方法。在 102 名变异携带者中鉴定出 16 种血红蛋白变异。最常见的变异是 Hb E，其次是 Hb Q-Thailand、Hb New York 和 Hb J-Bangkok。Capillarys 3 OCTA（两种 Hb 变异）、ADAMS HA-8180V（七种 Hb 变异）、HLC-723 G8（14 种 Hb 变异）和 Cobas c501（两种 Hb 变异）均观察到临床显著干扰。Capillarys 3 OCTA 的不可接受 HbA1c 结果比例为 13.7%，HA-8180V 为 52.9%，HLC-723 G8 为 83.3%，Cobas c501 为 3.9%。中国的血红蛋白变异严重影响了一些常用 HbA1c 方法的准确性。
