Clinical Medical Laboratory of Peking University Shenzhen Hospital, Shenzhen, Guangdong, P.R.China.
Clinical Medical Laboratory of Peking University Shenzhen Hospital, Lianhua Road No. 1120, Futian District, Shenzhen, Guangdong, P.R.China, Phone/Fax: +86-0755-83923333-2299.
Clin Chem Lab Med. 2018 Jul 26;56(8):1353-1361. doi: 10.1515/cclm-2017-1211.
HbA1c is a widely used biomarker for diabetes mellitus management. Here, we evaluated the accuracy of six methods for determining HbA1c values in Chinese patients with common α- and β-globin chains variants in China.
Blood samples from normal subjects and individuals exhibiting hemoglobin variants were analyzed for HbA1c, using Sebia Capillarys 2 Flex Piercing (C2FP), Bio-Rad Variant II Turbo 2.0, Tosoh HLC-723 G8 (ver. 5.24), Arkray ADAMS A1c HA-8180V fast mode, Cobas c501 and Trinity Ultra2 systems.
DNA sequencing revealed five common β-globin chain variants and three common α-globin chain variants. The most common variant was Hb E, followed by Hb New York, Hb J-Bangkok, Hb G-Coushatta, Hb Q-Thailand, Hb G-Honolulu, Hb Ube-2 and Hb G-Taipei. Variant II Turbo 2.0, Ultra2 and Cobas c501 showed good agreement with C2FP for most samples with variants. HLC-723 G8 yielded no HbA1c values for Hb J-Bangkok, Hb Q-Thailand and Hb G-Honolulu. Samples with Hb E, Hb G-Coushatta, Hb G-Taipei and Hb Ube-2 produced significant negative biases for HLC-723 G8. HA-8180V showed statistically significant differences for Hb E, Hb G-Coushatta, Hb G-Taipei, Hb Q-Thailand and Hb G-Honolulu. HA-8180V yielded no HbA1c values for Hb J-Bangkok. All methods showed good agreement for samples with Hb New York.
Some common hemoglobin variants can interfere with HbA1c determination by the most popular methods in China.
HbA1c 是糖尿病管理中广泛使用的生物标志物。在这里，我们评估了六种方法在我国常见的α和β珠蛋白链变异的中国患者中测定 HbA1c 值的准确性。
使用 Sebia Capillarys 2 Flex Piercing（C2FP）、Bio-Rad Variant II Turbo 2.0、Tosoh HLC-723 G8（ver. 5.24）、Arkray ADAMS A1c HA-8180V 快速模式、Cobas c501 和 Trinity Ultra2 系统分析正常受试者和血红蛋白变异个体的血液样本中的 HbA1c。
DNA 测序揭示了五种常见的β珠蛋白链变异和三种常见的α珠蛋白链变异。最常见的变异是 Hb E，其次是 Hb New York、Hb J-Bangkok、Hb G-Coushatta、Hb Q-Thailand、Hb G-Honolulu、Hb Ube-2 和 Hb G-Taipei。Variant II Turbo 2.0、Ultra2 和 Cobas c501 对大多数变异样本与 C2FP 具有良好的一致性。HLC-723 G8 对 Hb J-Bangkok、Hb Q-Thailand 和 Hb G-Honolulu 未产生 HbA1c 值。Hb E、Hb G-Coushatta、Hb G-Taipei 和 Hb Ube-2 的样本对 HLC-723 G8 产生显著的负偏倚。HA-8180V 对 Hb E、Hb G-Coushatta、Hb G-Taipei、Hb Q-Thailand 和 Hb G-Honolulu 存在统计学差异。HA-8180V 对 Hb J-Bangkok 未产生 HbA1c 值。所有方法对 Hb New York 样本均具有良好的一致性。
在中国最流行的方法中，一些常见的血红蛋白变异可能会干扰 HbA1c 的测定。
