Tjempakasari Artaria, Suroto Heri, Santoso Djoko
Doctoral Program of Medical Science, Faculty of Medicine, Universitas Airlangga, Indonesia.
Department of Internal Medicine, Faculty of Medicine, Universitas Airlangga - Dr. Soetomo General Academic Hospital, Surabaya, Indonesia.
Ann Med Surg (Lond). 2022 Oct 27;84:104796. doi: 10.1016/j.amsu.2022.104796. eCollection 2022 Dec.
Chronic kidney disease (CKD) is a health problem associated with high morbidity and mortality. Mineral and bone disorders are complications of CKD with a risk of fractures and cardiovascular disease. Mesenchymal stem cells can differentiate into osteoblasts and regulate their regulation by a network of cytokines and transcription factors.
Analyzing differences in osteoblastogenesis of adipose mesenchymal stem cells in CKD patients and healthy people.
The study sample was adipose mesenchymal stem cells from CKD patient undergoing hemodialysis and healthy people. Osteoblastogenesis was assessed by measuring the concentrations of transforming growth factor-β1 (TGF-β1), bone morphogenetic protein-2 (BMP-2), and (DKK-1) in culture media. The Elisa method measured the concentration of these parameters on days 4, 7, 14, and 21. Data were analyzed using an independent -test and post hoc test with -value <0.05.
There was a significant difference in CKD patients with increasing TGF-β1 on day 4 ( = 2.821; 95% CI = 30,498-199,727; = 0.010) and decreased on day 14. In the BMP-2 parameter, there was an increase on day 7 ( = 4.291; 95% CI = 0.289-0.831; <0.001). Similar conditions were also found in the DKK-1 parameter, increasing on the 7th day, but there was no significant difference ( = 0.583).
Osteoblastogenesis in adipose mesenchymal stem cells in CKD patients differs from that in healthy individuals. Osteoblasts fail in maturation and cause failure in matrix mineralization.
慢性肾脏病（CKD）是一个与高发病率和死亡率相关的健康问题。矿物质和骨代谢紊乱是CKD的并发症，存在骨折和心血管疾病风险。间充质干细胞可分化为成骨细胞，并通过细胞因子和转录因子网络对其进行调控。
分析CKD患者与健康人脂肪间充质干细胞成骨分化的差异。
研究样本为接受血液透析的CKD患者和健康人的脂肪间充质干细胞。通过测量培养基中转化生长因子-β1（TGF-β1）、骨形态发生蛋白-2（BMP-2）和 Dickkopf-1（DKK-1）的浓度来评估成骨分化。酶联免疫吸附测定（ELISA）法在第4、7、14和21天测量这些参数的浓度。采用独立样本t检验和事后检验分析数据，P值<0.05。
CKD患者在第4天TGF-β1升高（t = 2.821；95%置信区间= 30498 - 199727；P = 0.010），在第14天降低，差异有统计学意义。在BMP-2参数方面，第7天升高（t = 4.291；95%置信区间= 0.289 - 0.831；P < 0.001）。DKK-1参数也有类似情况，在第7天升高，但差异无统计学意义（t = 0.583）。
CKD患者脂肪间充质干细胞的成骨分化与健康个体不同。成骨细胞成熟失败并导致基质矿化失败。
