Osteoblastogenesis of adipose-derived mesenchymal stem cells in chronic kidney disease patient with regular hemodialysis.

BACKGROUND

Chronic kidney disease (CKD) is a health problem associated with high morbidity and mortality. Mineral and bone disorders are complications of CKD with a risk of fractures and cardiovascular disease. Mesenchymal stem cells can differentiate into osteoblasts and regulate their regulation by a network of cytokines and transcription factors.

OBJECTIVE

Analyzing differences in osteoblastogenesis of adipose mesenchymal stem cells in CKD patients and healthy people.

METHODS

The study sample was adipose mesenchymal stem cells from CKD patient undergoing hemodialysis and healthy people. Osteoblastogenesis was assessed by measuring the concentrations of transforming growth factor-β1 (TGF-β1), bone morphogenetic protein-2 (BMP-2), and (DKK-1) in culture media. The Elisa method measured the concentration of these parameters on days 4, 7, 14, and 21. Data were analyzed using an independent -test and post hoc test with -value <0.05.

RESULT

There was a significant difference in CKD patients with increasing TGF-β1 on day 4 ( = 2.821; 95% CI = 30,498-199,727;  = 0.010) and decreased on day 14. In the BMP-2 parameter, there was an increase on day 7 ( = 4.291; 95% CI = 0.289-0.831;  <0.001). Similar conditions were also found in the DKK-1 parameter, increasing on the 7th day, but there was no significant difference ( = 0.583).

CONCLUSION

Osteoblastogenesis in adipose mesenchymal stem cells in CKD patients differs from that in healthy individuals. Osteoblasts fail in maturation and cause failure in matrix mineralization.