Yoshida Shohei, Yoshida Masashi, Wéber György, Csukás Domokos, Blázovics Anna, Szabó Györgyi, Sándor József, Ohdaira Hironori, Suzuki Yutaka, Ferencz Andrea
Department of Surgical Research and Techniques, Semmelweis University, Budapest, Hungary.
Department of Surgery, International University of Health and Welfare Hospital, Tochigi, Japan.
Ann Med Surg (Lond). 2022 Nov 15;84:104923. doi: 10.1016/j.amsu.2022.104923. eCollection 2022 Dec.
A significant difference exists between the reported optimal timing of indocyanine green (ICG) injection during fluorescence cholangiography and ICG dissipation time from the serum. There are no reports on alterations in ICG concentration in biliary fluid over time. Herein, we measured the concentration of ICG and the fluorescence intensity ratio between the common bile duct (CBD) and liver, which was recognized as a parameter of the visibility of the CBD.
ICG (0.05 mg/kg) was injected intravenously into female pigs (n = 7). Afterwards, the fluorescence of the CBD and liver was detected at 30 min, 2 h, and 4 h. Biliary fluid was collected from cannulated CBD tubes. The fluorescence intensity was measured using captured images and calculated using the ImageJ image-processing program. ICG concentration was measured using spectrophotometry and compared using an analysis of variance test.
Biliary ICG concentrations at 30 min, 2 h, and 4 h were 92.07 ± 27.72 μg/mL, 37.14 ± 9.76 μg/mL (p < 0.05 vs. 30 min), and 13.91 ± 5.71 μg/mL (p < 0.05 vs. 30 min), respectively; p < 0.01. The CBD/liver fluorescence intensity ratios at 30 min, 2 h, and 4 h were 1.25 ± 0.72, 2.39 ± 1.28 (p < 0.05 vs. 30 min and 4 h), and 3.38 ± 1.73 (p < 0.05 vs. 30 min and 2 h), respectively.
The ICG biliary concentration was highest at 30 min, whereas the CBD/liver fluorescence intensity ratio was highest at 4 h. Decreasing the fluorescence intensity of the liver may be an important approach for improving the visualization of the CBD during fluorescence cholangiography.
PE/EA/491-5/2020.
荧光胆管造影术中吲哚菁绿（ICG）注射的最佳时机与ICG从血清中的消散时间之间存在显著差异。目前尚无关于胆汁中ICG浓度随时间变化的报道。在此，我们测量了ICG的浓度以及胆总管（CBD）与肝脏之间的荧光强度比，该比值被视为胆总管可视性的一个参数。
将ICG（0.05mg/kg）静脉注射到雌性猪（n = 7）体内。之后，在30分钟、2小时和4小时检测胆总管和肝脏的荧光。从插入胆总管的导管中收集胆汁。使用捕获的图像测量荧光强度，并使用ImageJ图像处理程序进行计算。使用分光光度法测量ICG浓度，并通过方差分析进行比较。
30分钟、2小时和4小时时胆汁中ICG浓度分别为92.07±27.72μg/mL、37.14±9.76μg/mL（与30分钟相比，p < 0.05）和13.91±5.71μg/mL（与30分钟相比，p < 0.05）；p < 0.01。30分钟、2小时和4小时时CBD/肝脏荧光强度比分别为1.25±0.72、2.39±1.28（与30分钟和4小时相比，p < 0.05）和3.38±1.73（与30分钟和2小时相比，p < 0.05）。
ICG胆汁浓度在30分钟时最高，而CBD/肝脏荧光强度比在4小时时最高。降低肝脏的荧光强度可能是在荧光胆管造影术中改善胆总管可视化的重要方法。
PE/EA/491 - 5/2020。
