Sakthivel Krishnan, Manigundan Kaari, Sharma Sushil Kumar, Singh Reena, Das Manisha Mohan, Devi Varsha, Gautam Raj Kumar, Nakkeeran Sevugapperumal, Kumar Aundy
Division of Field Crop Improvement and Protection, ICAR- Central Islands Agricultural Research Institute, Port Blair, Andaman & Nicobar Islands, 744 101, India.
Centre for Drug Discovery and Development, Sathyabama Institute of Science and Technology, Chennai, 600 119, India.
Curr Microbiol. 2022 Dec 20;80(1):45. doi: 10.1007/s00284-022-03086-y.
Taxonomic and functional characterization of a total of 90 bacterial isolates representing bulk and rhizosphere soils of diverse niches of Andaman and Nicobar Islands, India were carried out. Twelve bacterial isolates were found promising for the biological suppression of agriculturally important fungal and bacterial plant pathogens such as Ralstonia solanacearum, Xanthomonas oryzae pv. oryzae, and Colletotrichum gloeosporioides. The 16S rRNA gene sequence analysis revealed their identity as belonging to Bacillus subtilis, Bacillus amyloliquefaciens, and Lysinibacillus sphaericus. The isolates were positive for plant growth promotion (PGP) traits including siderophore production, and nutrient solubilization especially phosphorous, zinc, and potassium. Interestingly, the PCR test confirmed the presence of 62 antimicrobial peptides (AMP) biosynthesis genes specific to the genus Bacillus. Whilst all tested species of Bacillus harboured the bacD biosynthesis gene, the B. subtilis (Ba_Abi), and B. amyloliquefaciens (Ba_Abi) harboured the maximum AMP biosynthesis genes analysed in the study. Upon in planta evaluation, the biocontrol potential of the bacterial isolates against leaf spot disease of chilli was observed. The study culminated in the isolation and identification of diverse Bacillus species for exploitation as bioinoculants for plant health management programmes.
对来自印度安达曼和尼科巴群岛不同生态位的大量土壤和根际土壤中的90株细菌分离株进行了分类和功能表征。发现12株细菌分离株有望对农业上重要的真菌和细菌植物病原体，如青枯雷尔氏菌、水稻黄单胞菌稻瘟病菌和炭疽菌进行生物抑制。16S rRNA基因序列分析表明它们分别属于枯草芽孢杆菌、解淀粉芽孢杆菌和球形赖氨酸芽孢杆菌。这些分离株在促进植物生长（PGP）特性方面呈阳性，包括铁载体产生以及养分溶解，尤其是磷、锌和钾。有趣的是，PCR检测证实存在62个特定于芽孢杆菌属的抗菌肽（AMP）生物合成基因。虽然所有测试的芽孢杆菌物种都含有bacD生物合成基因，但枯草芽孢杆菌（Ba_Abi）和解淀粉芽孢杆菌（Ba_Abi）含有该研究中分析的最多的AMP生物合成基因。在植物体内评估时，观察到这些细菌分离株对辣椒叶斑病的生物防治潜力。该研究最终分离并鉴定了多种芽孢杆菌物种，以用作植物健康管理计划的生物接种剂。
