Javed M A, Ali S W, Ashfaq M, Tabassam J, Ali M, IhsanUllah M, Nayab S F, Kaya Y, Khalili E, Ali Q, Yau T E
University of the Punjab, Faculty of Agricultural Sciences, Department of Plant Breeding and Genetics, Lahore, Pakistan.
Universiti Teknologi Malaysia - UTM Skudai, Faculty of Science, Department of Biosciences, Johor Bahru, Malaysia.
Braz J Biol. 2022 Dec 16;82:e256189. doi: 10.1590/1519-6984.256189. eCollection 2022.
Bacteria blight is one of the most serious bacterial diseases of rice worldwide. The identification of genetic potential against bacterial blight in the existing rice resources is a prerequisite to develop multigenic resistance to combat the threat of climate change. This investigation was conducted to evaluate alleles variation in 38 Malaysian cultivars using thirteen Simple Sequences Repeats markers and one Sequence Tagged Sites (STS) marker which were reported to be linked with the resistance to bacterial blight. Based on molecular data, a dendrogram was constructed which classified the rice cultivars into seven major clusters at 0.0, 0.28 and 0.3 of similarity coefficient. Cluster 5 was the largest group comprised of ten rice cultivars where multiple genes were identified. However, xa13 could not be detected in the current rice germplasm, whereas xa2 was detected in 25 cultivars. Molecular analysis revealed that Malaysian rice cultivars possess multigenic resistance.
白叶枯病是全球最严重的水稻细菌性病害之一。鉴定现有水稻资源中抗白叶枯病的遗传潜力是培育多基因抗性以应对气候变化威胁的先决条件。本研究利用13个简单序列重复标记和1个序列标签位点(STS)标记评估了38个马来西亚品种的等位基因变异,这些标记据报道与抗白叶枯病有关。基于分子数据构建了一个树状图,该图在相似系数为0.0、0.28和0.3时将水稻品种分为7个主要类群。第5类是最大的组,由10个水稻品种组成,其中鉴定出了多个基因。然而,在当前的水稻种质中未检测到xa13,而在25个品种中检测到了xa2。分子分析表明,马来西亚水稻品种具有多基因抗性。