Department of Molecular Bacteriology, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamada-oka, Suita, Osaka 565-0871, Japan.
Food Hygiene and Environmental Health, Division of Applied Life Science, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, 1-5 Shimogamohangi-cho, Sakyo-ku, Kyoto 606-8522, Japan.
Sci Adv. 2022 Dec 21;8(51):eade8971. doi: 10.1126/sciadv.ade8971.
Bacterial small RNAs (sRNAs) posttranscriptionally regulate gene expressions involved in various biological processes, including pathogenicity. Our previous study identified sRNAs, the expression of which was up-regulated in , the causative agent of whooping cough, upon tracheal colonization of the bacteria; however, their roles in bacterial infection remain unknown. Here, we found that one sRNA, Bpr4, contributes to infection by posttranscriptionally up-regulating filamentous hemagglutinin (FHA), a major adhesin of the bacteria. Bpr4 bound to the 5' untranslated region of mRNA encoding FHA and inhibited its degradation mediated by RNaseE. Our results demonstrated that Bpr4 up-regulation was triggered by the interference of flagellar rotation, which caused the disengagement of MotA, a flagellar stator. Subsequently, MotA activated a diguanylate cyclase to generate cyclic di-GMP, which plays a role in Bpr4 up-regulation through the RisK/RisA two-component system. Our findings indicate that a flagellum-triggered sensory system contributes to infection.
细菌小 RNA（sRNA）通过转录后调控参与各种生物学过程的基因表达，包括致病性。我们之前的研究发现，在引起百日咳的细菌气管定植时，其表达上调的 sRNA；然而，它们在细菌感染中的作用尚不清楚。在这里，我们发现一种 sRNA，Bpr4，通过转录后上调丝状血凝素（FHA）来促进 感染，FHA 是细菌的主要黏附素。Bpr4 与编码 FHA 的 mRNA 的 5'非翻译区结合，并抑制其由 RNaseE 介导的降解。我们的结果表明，Bpr4 的上调是由鞭毛旋转的干扰触发的，这导致鞭毛定子 MotA 脱离。随后，MotA 激活二鸟苷酸环化酶产生环二鸟苷酸，该物质通过 RisK/RisA 双组分系统在 Bpr4 的上调中发挥作用。我们的研究结果表明，一个鞭毛触发的感应系统有助于 感染。
Zotero 插件
