State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, 430062, China.
School of Forestry and Biotechnology, Zhejiang A & F University, National Joint Local Engineering Laboratory for High-Efficient Preparation of Biopesticide, Lin'an, 311300, China.
BMC Genomics. 2022 Dec 22;23(1):845. doi: 10.1186/s12864-022-09079-2.
In insects, the chemosensory system is crucial in guiding their behaviors for survival. Plagiodera versicolora (Coleoptera: Chrysomelidae), is a worldwide leaf-eating forest pest in salicaceous trees. There is little known about the chemosensory genes in P. versicolora. Here, we conducted a transcriptome analysis of larvae heads in P. versicolora.
In this study, 29 odorant binding proteins (OBPs), 6 chemosensory proteins (CSPs), 14 odorant receptors (ORs), 13 gustatory receptors (GRs), 8 ionotropic receptors (IRs) and 4 sensory neuron membrane proteins (SNMPs) were identified by transcriptome analysis. Compared to the previous antennae and foreleg transcriptome data in adults, 12 OBPs, 2 CSPs, 5 ORs, 4 IRs, and 7 GRs were newly identified in the larvae. Phylogenetic analyses were conducted and found a new candidate CO receptor (PverGR18) and a new sugar receptor (PverGR23) in the tree of GRs. Subsequently, the dynamic expression profiles of various genes were analyzed by quantitative real-time PCR. The results showed that PverOBP31, OBP34, OBP35, OBP38, and OBP40 were highly expressed in larvae, PverOBP33 and OBP37 were highly expressed in pupae, and PverCSP13 was highly expressed in eggs, respectively.
We identified a total of 74 putative chemosensory genes based on a transcriptome analysis of larvae heads in P. versicolora. This work provides new information for functional studies on the chemoreception mechanism in P. versicolora.
在昆虫中，化学感觉系统对其生存行为的指导至关重要。白杨潜叶蛾（Coleoptera：Chrysomelidae）是一种全球性的食叶性森林害虫，以杨柳科树木为食。目前，对白杨潜叶蛾的化学感觉基因知之甚少。在这里，我们对白杨潜叶蛾幼虫头部进行了转录组分析。
在这项研究中，通过转录组分析鉴定了 29 个气味结合蛋白（OBP）、6 个化学感觉蛋白（CSP）、14 个气味受体（OR）、13 个味觉受体（GR）、8 个离子型受体（IR）和 4 个感觉神经元膜蛋白（SNMP）。与之前成虫触角和前腿转录组数据相比，在幼虫中鉴定出 12 个 OBP、2 个 CSP、5 个 OR、4 个 IR 和 7 个 GR 为新基因。系统发育分析发现，在 GR 树中存在一个新的候选 CO 受体（PverGR18）和一个新的糖受体（PverGR23）。随后，通过定量实时 PCR 分析了各种基因的动态表达谱。结果表明，PverOBP31、OBP34、OBP35、OBP38 和 OBP40 在幼虫中高度表达，PverOBP33 和 OBP37 在蛹中高度表达，PverCSP13 在卵中高度表达。
我们基于白杨潜叶蛾幼虫头部的转录组分析，共鉴定出 74 个潜在的化学感觉基因。这项工作为白杨潜叶蛾化学感觉机制的功能研究提供了新的信息。
