College of Agriculture / Key Laboratory of Oasis Agricultural Pest Management and Plant Protection Resources Utilization, Xinjiang Uygur Autonomous Region, Shihezi University, Shihezi, Xinjiang, China.
Yuli Industry Development Service Center of Apocynum venetum, Xinjiang, China.
PLoS One. 2024 Jun 7;19(6):e0301177. doi: 10.1371/journal.pone.0301177. eCollection 2024.
In the polyphagous insect Monolepta signata (M. signata) (Coleoptera: Chrysomelidae), antennae are important for olfactory reception used during feeding, mating, and finding a suitable oviposition site. Based on NextSeq 6000 Illumina sequencing, we assembled the antennal transcriptome of mated M. signata and described the first chemosensory gene repertoire expressed in this species. The relative expression levels of some significant chemosensory genes were conducted by quantitative real-time PCR. We identified 114 olfactory-related genes based on the antennal transcriptome database of M. signata, including 21 odorant binding proteins (OBPs), six chemosensory proteins (CSPs), 46 odorant receptors (ORs), 15 ionotropic receptors (IRs), 23 gustatory receptors (GRs) and three sensory neuron membrane proteins (SNMPs). Blastp best hit and phylogenetic analyses showed that most of the chemosensory genes had a close relationship with orthologs from other Coleoptera species. Overall, this study provides a foundation for elucidating the molecular mechanism of olfactory recognition in M. signata as well as a reference for the study of chemosensory genes in other species of Coleoptera.
在多食性昆虫 Monolepta signata（M. signata）（鞘翅目：叶甲科）中，触角在取食、交配和寻找合适产卵场所时的嗅觉接收中起着重要作用。基于 NextSeq 6000 Illumina 测序，我们组装了交配的 M. signata 的触角转录组，并描述了该物种中首次表达的化学感觉基因库。通过定量实时 PCR 检测了一些重要化学感觉基因的相对表达水平。我们根据 M. signata 的触角转录组数据库鉴定了 114 个嗅觉相关基因，包括 21 个气味结合蛋白（OBPs）、6 个化学感觉蛋白（CSPs）、46 个气味受体（ORs）、15 个离子型受体（IRs）、23 个味觉受体（GRs）和 3 个感觉神经元膜蛋白（SNMPs）。Blastp 最佳命中和系统发育分析表明，大多数化学感觉基因与其他鞘翅目物种的同源物密切相关。总的来说，这项研究为阐明 M. signata 嗅觉识别的分子机制提供了基础，也为其他鞘翅目物种化学感觉基因的研究提供了参考。
