油菜素内酯信号与katanin 介导的微管切割合作控制雄蕊花丝伸长。
Brassinosteroid signals cooperate with katanin-mediated microtubule severing to control stamen filament elongation.
机构信息
State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
University of Chinese Academy of Sciences, Beijing, China.
出版信息
EMBO J. 2023 Feb 15;42(4):e111883. doi: 10.15252/embj.2022111883. Epub 2022 Dec 22.
Proper stamen filament elongation is essential for pollination and plant reproduction. Plant hormones are extensively involved in every stage of stamen development; however, the cellular mechanisms by which phytohormone signals couple with microtubule dynamics to control filament elongation remain unclear. Here, we screened a series of Arabidopsis thaliana mutants showing different microtubule defects and revealed that only those unable to sever microtubules, lue1 and ktn80.1234, displayed differential floral organ elongation with less elongated stamen filaments. Prompted by short stamen filaments and severe decrease in KTN1 and KTN80s expression in qui-2 lacking five BZR1-family transcription factors (BFTFs), we investigated the crosstalk between microtubule severing and brassinosteroid (BR) signaling. The BFTFs transcriptionally activate katanin-encoding genes, and the microtubule-severing frequency was severely reduced in qui-2. Taken together, our findings reveal how BRs can regulate cytoskeletal dynamics to coordinate the proper development of reproductive organs.
花丝的正常伸长对于授粉和植物繁殖至关重要。植物激素广泛参与雄蕊发育的各个阶段；然而，植物激素信号与微管动力学如何偶联以控制花丝伸长的细胞机制尚不清楚。在这里，我们筛选了一系列表现出不同微管缺陷的拟南芥突变体，并揭示只有那些不能切断微管的突变体lue1 和 ktn80.1234 显示出不同的花器官伸长，花丝伸长较短。由于 qui-2 缺乏五个 BZR1 家族转录因子（BFTFs），导致短花丝和 KTN1 和 KTN80s 表达严重下降，我们研究了微管切断和油菜素内酯（BR）信号之间的串扰。BFTFs 转录激活katanin 编码基因，并且微管切断频率在 qui-2 中严重降低。总之，我们的研究结果揭示了 BR 如何调节细胞骨架动力学以协调生殖器官的正常发育。
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