Department of Biomedical Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing, 210016, China.
The State Key Laboratory of Translational Medicine and Innovative Drug Development, Jiangsu Simcere pharmaceutical Co., Ltd, Nanjing, 210016, China.
BMC Genomics. 2022 Dec 22;23(Suppl 4):848. doi: 10.1186/s12864-022-09008-3.
The imbalance of intestinal flora may promote the occurrence and development of colorectal cancer, changes of the intestinal flora during the development of colorectal cancer and the mechanism that promotes the colorectal cancer were discovered in this study. Deep sequencing of the microbial 16 s ribosomal RNA gene was used to investigate alterations in feces samples of mice at the early inflammation stage and fully developed stage of colorectal cancer.
According to PCoA analysis and ANOSIM test, we found the intestinal flora had significantly changed in mice with colorectal inflammation or colorectal cancer compared with healthy mice (p < 0.05). Using correlation analysis, we found that Muribaculaceae and Bacteroidaceae had strong excluding interactions. The functional changes of the gut microbiota include the up-regulation of the cancers pathway and the down-regulation of the replication and repair pathways.
Our study found the intestinal flora of mice suffering from colorectal inflammation and colorectal cancer has changed significantly, especially the decrease of Muribaculaceae and the increase of Bacteroidaceae. We suppose that these two floras may play an important role in development of colorectal cancer.
肠道菌群失衡可能促进结直肠癌的发生发展，本研究旨在探讨结直肠癌发生发展过程中肠道菌群的变化及其促进结直肠癌发生的机制。采用微生物 16s 核糖体 RNA 基因深度测序的方法，研究了处于结直肠炎症早期和完全发展阶段的结直肠癌小鼠粪便样本中的变化。
根据 PCoA 分析和 ANOSIM 检验，我们发现与健康小鼠相比，患有结直肠炎症或结直肠癌的小鼠肠道菌群发生了显著变化（p<0.05）。通过相关性分析，我们发现 Muribaculaceae 和 Bacteroidaceae 具有很强的排斥相互作用。肠道微生物群的功能变化包括癌症途径的上调和复制及修复途径的下调。
本研究发现患有结直肠炎症和结直肠癌的小鼠肠道菌群发生了显著变化，特别是 Muribaculaceae 的减少和 Bacteroidaceae 的增加。我们推测这两种菌群可能在结直肠癌的发生发展中发挥重要作用。
