Fehaid Alaa, Al-Ghamdi Mohammad S, Alzahrani Khalid J, Theyab Abdulrahman, Al-Amer Osama M, Al-Shehri Saad S, Algahtani Mohammad, A Oyouni Atif Abdulwahab, Alnfiai Mrim M, Aly Mohamed H, Alsharif Khalaf F, Albrakati Ashraf, Kassab Rami B, Althagafi Hussam A, Alharthi Fahad, Abdel Moneim Ahmed E, Lokman Maha S
Forensic Medicine and Toxicology Department, Faculty of Veterinary Medicine, Mansoura University, Dakahlia, Egypt.
Department of Clinical Laboratories Sciences, College of Applied Medical Sciences, Taif University, Taif, Saudi Arabia.
J Biochem Mol Toxicol. 2023 Mar;37(3):e23275. doi: 10.1002/jbt.23275. Epub 2022 Dec 22.
Exposure to lead (Pb) is associated with serious health problems including hepatorenal toxicity. Apigenin is a natural-sourced flavonoid with promising antioxidant and anti-inflammatory effects. In this research, we investigated the potential protective role of apigenin against lead acetate (PbAc)-induced hepatorenal damage. Thus, this experiment studied the exposure of male Wistar Albino rats to apigenin and/or PbAc and their effects in comparison to the control rats. Apigenin administration decreased the levels of Pb and prevented the histopathological deformations in liver and kidney tissues following PbAc exposure. This was confirmed by the normalized levels of liver and kidney function markers. Additionally, apigenin inhibited significantly oxidative reactions through upregulating Nrf2 and HO-1, and activating their downstreamed antioxidants accompanied by a marked depletion of pro-oxidants. Moreover, apigenin decreased the elevated pro-inflammatory cytokines and inhibited cell loss in liver and kidney tissues in response to PbAc intoxication in both tissues. The obtained results demonstrated that apigenin could be used to attenuate the molecular, biochemical, and histological alterations associated with Pb exposure due to its potent antioxidant, anti-inflammatory, and antiapoptotic effects.
接触铅（Pb）会引发包括肝肾毒性在内的严重健康问题。芹菜素是一种具有潜在抗氧化和抗炎作用的天然来源黄酮类化合物。在本研究中，我们探究了芹菜素对醋酸铅（PbAc）诱导的肝肾损伤的潜在保护作用。因此，本实验研究了雄性Wistar白化大鼠接触芹菜素和/或PbAc的情况及其与对照大鼠相比的影响。给予芹菜素可降低铅水平，并防止PbAc暴露后肝脏和肾脏组织的组织病理学变形。肝脏和肾脏功能标志物水平的正常化证实了这一点。此外，芹菜素通过上调Nrf2和HO-1并激活其下游抗氧化剂，同时显著消耗促氧化剂，从而显著抑制氧化反应。此外，芹菜素降低了肝脏和肾脏组织中升高的促炎细胞因子，并抑制了两种组织中因PbAc中毒而导致的细胞损失。所得结果表明，芹菜素因其强大的抗氧化、抗炎和抗凋亡作用，可用于减轻与铅暴露相关的分子、生化和组织学改变。
