Department of Biochemistry, Faculty of Veterinary Medicine, Ataturk University, Erzurum, Turkey.
Department of Midwifery, Faculty of Health Science, Munzur University, Tunceli, Turkey.
J Food Biochem. 2021 Feb;45(2):e13593. doi: 10.1111/jfbc.13593. Epub 2020 Dec 27.
In the present study, the protective effects of chrysin (CHR) against testicular damage caused by lead acetate (PbAc) were examined. In this way, 30 min after rats were given 25 and 50 mg/kg/b.w CHR orally for seven consecutive days, 30 mg/kg/b.w PbAc was administered orally. In biochemical analysis of testicular tissue, it was found that PbAc-reduced antioxidant parameters [glutathione peroxidase (GPx), glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT)], while it increased lipid peroxidation, inflammatory markers [nuclear factor kappa-B (NF-κB), cyclooxygenase-2 (COX-2), prostaglandin E2 (PGE-2), and inducible nitric oxide synthase (iNOS)], and 8-hydroxy-2'-deoxyguanosine (8-OHdG). In the immunohistochemical examination, it was determined that PbAc increased the expression of tumor necrosis factor-α (TNF-α) and caspase-3. Accordingly, PbAc was found to cause a decrease in sperm motility and an increase in the percentage of dead sperm. However, it has been observed that CHR relieves oxidative stress due to its antioxidant properties, thus protecting against inflammation and apoptosis. It also allowed the CHR sperm parameters to return to control group levels. The results revealed that CHR could be a natural substance to be used in Pb-induced testicular toxicity. PRACTICAL APPLICATIONS: Lead (Pb) is an important environmental contaminant heavy metal. Pb is believed to reduce fertility in men. Oxidative stress plays a significant role in the damage caused by Pb to testicular tissue. CHR is an antioxidant substance that occurs naturally in various plants and has various pharmacological properties. In the present study, it was investigated whether CHR has a protective effect against testicular toxicity induced by PbAc. The results revealed that in rats, CHR protects the testicular tissue from PbAc toxicity by showing antioxidant, anti-inflammatory and anti-apoptotic effects, thus bringing sperm parameters closer to normal.
在本研究中，研究了白杨素（CHR）对醋酸铅（PbAc）引起的睾丸损伤的保护作用。为此，大鼠连续 7 天每天口服 25 和 50mg/kg/b.w CHR30 分钟后，口服 30mg/kg/b.w PbAc。在睾丸组织的生化分析中，发现 PbAc 降低了抗氧化参数[谷胱甘肽过氧化物酶（GPx）、谷胱甘肽（GSH）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）]，而增加了脂质过氧化、炎症标志物[核因子 kappa-B（NF-κB）、环氧化酶-2（COX-2）、前列腺素 E2（PGE-2）和诱导型一氧化氮合酶（iNOS）]和 8-羟基-2'-脱氧鸟苷（8-OHdG）。在免疫组织化学检查中，确定 PbAc 增加了肿瘤坏死因子-α（TNF-α）和半胱天冬酶-3 的表达。因此，PbAc 导致精子活力降低和死精子比例增加。然而，已经观察到 CHR 由于其抗氧化特性缓解了氧化应激，从而防止了炎症和细胞凋亡。它还使 CHR 的精子参数恢复到对照组水平。结果表明，CHR 可以作为一种天然物质，用于治疗 Pb 引起的睾丸毒性。实际应用：铅（Pb）是一种重要的环境污染物重金属。人们认为 Pb 会降低男性的生育能力。氧化应激在 Pb 对睾丸组织造成的损伤中起着重要作用。CHR 是一种天然存在于各种植物中的抗氧化物质，具有多种药理学特性。在本研究中，研究了 CHR 是否对 PbAc 引起的睾丸毒性具有保护作用。结果表明，在大鼠中，CHR 通过表现出抗氧化、抗炎和抗凋亡作用，保护睾丸组织免受 PbAc 毒性的侵害，从而使精子参数更接近正常。
