• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

基于超支化环糊精的聚合物作为抗凝血剂：体外和体内研究

Hyper-Branched Cyclodextrin-Based Polymers as Anticoagulant Agents: In Vitro and In Vivo Studies.

作者信息

Monfared Yousef Khazaei, Mahmoudian Mohammad, Hoti Gjylije, Bisericaru Daniel Mihai, Caldera Fabrizio, Cavalli Roberta, Zakeri-Milani Parvin, Matencio Adrián, Trotta Francesco

机构信息

Dipartimento di Chimica and NIS, Università di Torino, Via P. Giuria 7, 10125 Torino, Italy.

Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz 5166-15731, Iran.

出版信息

Bioengineering (Basel). 2022 Dec 4;9(12):765. doi: 10.3390/bioengineering9120765.

DOI:10.3390/bioengineering9120765
PMID:36550971
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9774232/
Abstract

This study tested the anticoagulant effect of cyclodextrin (CD) hyper-branched-based polymers (HBCD-Pols). These polymers were synthesized and tested for their coagulant characteristics in vitro and in vivo. Due to their polymeric structure and anionic nature, the polymers can chelate Ca, reducing the free quantity in blood. HBCD-Pol increased the blood clotting time, PT, and aPTT 3.5 times over the control, showing a better effect than even ethylenediaminetetraacetic acid (EDTA), as occured with recalcification time as well. A titration of HBCD-Pol and EDTA showed exciting differences in the ability to complex Ca between both materials. Before executing in vivo studies, a hemocompatibility study was carried out with less than 5% red blood cell hemolysis. The fibrinogen consumption and bleeding time were analyzed in vivo. The fibrinogen was considerably decreased in the presence of HBCD-Pol in a higher grade than EDTA, while the bleeding time was longer with HBCD-Pols. The results demonstrate that the anticoagulant effect of this HBCD-Pol opens novel therapy possibilities due to the possible transport of drugs in this carrier. This would give combinatorial effects and a potential novel anticoagulant therapy with HBCD-Pol per se.

摘要

本研究测试了基于环糊精（CD）超支化聚合物（HBCD-Pols）的抗凝效果。合成了这些聚合物，并在体外和体内测试了它们的凝血特性。由于其聚合物结构和阴离子性质，这些聚合物可以螯合钙，减少血液中的游离钙量。HBCD-Pol使血液凝固时间、PT和活化部分凝血活酶时间（aPTT）比对照组延长了3.5倍，甚至比乙二胺四乙酸（EDTA）的效果更好，再钙化时间也是如此。HBCD-Pol和EDTA的滴定显示出两种材料在螯合钙能力上令人兴奋的差异。在进行体内研究之前，进行了红细胞溶血率低于5%的血液相容性研究。在体内分析了纤维蛋白原消耗和出血时间。在HBCD-Pol存在的情况下，纤维蛋白原的降低程度比EDTA更高，而HBCD-Pols的出血时间更长。结果表明，这种HBCD-Pol的抗凝作用由于药物可能在该载体中运输而开辟了新的治疗可能性。这将产生协同效应，并可能带来一种基于HBCD-Pol本身的新型抗凝疗法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f3/9774232/9c9eb22b2d47/bioengineering-09-00765-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f3/9774232/23f763a881fc/bioengineering-09-00765-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f3/9774232/c2e9d14eb386/bioengineering-09-00765-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f3/9774232/4240a0458224/bioengineering-09-00765-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f3/9774232/3d950575ab4a/bioengineering-09-00765-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f3/9774232/b5e0bfebb164/bioengineering-09-00765-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f3/9774232/a56a09c32545/bioengineering-09-00765-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f3/9774232/76cc1b8229c6/bioengineering-09-00765-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f3/9774232/9c9eb22b2d47/bioengineering-09-00765-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f3/9774232/23f763a881fc/bioengineering-09-00765-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f3/9774232/c2e9d14eb386/bioengineering-09-00765-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f3/9774232/4240a0458224/bioengineering-09-00765-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f3/9774232/3d950575ab4a/bioengineering-09-00765-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f3/9774232/b5e0bfebb164/bioengineering-09-00765-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f3/9774232/a56a09c32545/bioengineering-09-00765-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f3/9774232/76cc1b8229c6/bioengineering-09-00765-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f3/9774232/9c9eb22b2d47/bioengineering-09-00765-g008.jpg

相似文献

1
Hyper-Branched Cyclodextrin-Based Polymers as Anticoagulant Agents: In Vitro and In Vivo Studies.基于超支化环糊精的聚合物作为抗凝血剂：体外和体内研究
Bioengineering (Basel). 2022 Dec 4;9(12):765. doi: 10.3390/bioengineering9120765.
2
Evaluation of highly branched cyclic dextrin in inhalable particles of combined antibiotics for the pulmonary delivery of anti-tuberculosis drugs.用于抗结核药物肺部递送的联合抗生素可吸入颗粒中高度支化环糊精的评价
Int J Pharm. 2017 Jan 30;517(1-2):8-18. doi: 10.1016/j.ijpharm.2016.11.060. Epub 2016 Nov 29.
3
Purification and characterization of a novel thermostable anticoagulant protein from medicinal leech Whitmania pigra Whitman.从医用水蛭 Whitmania pigra Whitman 中纯化和表征一种新型耐热抗凝蛋白。
J Ethnopharmacol. 2022 Apr 24;288:114990. doi: 10.1016/j.jep.2022.114990. Epub 2022 Jan 13.
4
RNA Loading on Nano-Structured Hyperbranched β-Cyclodextrin.纳米结构超支化β-环糊精上的RNA负载
Avicenna J Med Biotechnol. 2018 Jan-Mar;10(1):15-21.
5
Evaluation of the main coagulation tests in the presence of hemolysis in healthy subjects and patients on oral anticoagulant therapy.健康受试者和接受口服抗凝治疗患者出现溶血时主要凝血试验的评估。
Int J Lab Hematol. 2015 Dec;37(6):819-33. doi: 10.1111/ijlh.12417. Epub 2015 Aug 25.
6
In vitro effects of detergent sclerosants on coagulation, platelets and microparticles.去污硬化剂对凝血、血小板和微粒的体外作用。
Eur J Vasc Endovasc Surg. 2007 Dec;34(6):731-40. doi: 10.1016/j.ejvs.2007.07.011. Epub 2007 Oct 4.
7
Parallel in vivo and in vitro transcriptomics analysis reveals calcium and zinc signalling in the brain as sensitive targets of HBCD neurotoxicity.平行的体内和体外转录组学分析揭示了钙和锌信号在大脑中作为 HBCD 神经毒性的敏感靶点。
Arch Toxicol. 2018 Mar;92(3):1189-1203. doi: 10.1007/s00204-017-2119-2. Epub 2017 Nov 25.
8
Coagulation Testing in the Core Laboratory.核心实验室中的凝血检测
Lab Med. 2017 Nov 8;48(4):295-313. doi: 10.1093/labmed/lmx050.
9
The role of ethylenediamine tetraacetic acid (EDTA) as in vitro anticoagulant for diagnostic purposes.乙二胺四乙酸（EDTA）作为用于诊断目的的体外抗凝剂的作用。
Clin Chem Lab Med. 2007;45(5):565-76. doi: 10.1515/CCLM.2007.110.
10
Underestimation of plasma level of factor V coagulant activity and fibrinogen concentration together with prolonged prothrombin time, activated partial thromboplastin time and thrombin time can result from pre-analytical very low calcium level in citrated sample tube.枸橼酸盐样本管中分析前钙水平极低，可能导致凝血因子V促凝活性的血浆水平和纤维蛋白原浓度被低估，同时凝血酶原时间、活化部分凝血活酶时间和凝血酶时间延长。
Int J Lab Hematol. 2016 Feb;38(1):50-3. doi: 10.1111/ijlh.12434. Epub 2015 Sep 25.

引用本文的文献

1
Mixed β-γ-Cyclodextrin Branched Polymer with Multiple Photo-Chemotherapeutic Cargos.具有多种光化学治疗药物的混合β-γ-环糊精支化聚合物
ACS Appl Polym Mater. 2023 Aug 31;5(10):7918-7926. doi: 10.1021/acsapm.3c01157. eCollection 2023 Oct 13.
2
Could the Convergence of Science and Technology Guarantee Human Health in the Future?科技的融合能否保障未来人类的健康？
Bioengineering (Basel). 2023 May 13;10(5):589. doi: 10.3390/bioengineering10050589.

本文引用的文献

1
Identification of a βCD-Based Hyper-Branched Negatively Charged Polymer as HSV-2 and RSV Inhibitor.基于 βCD 的超支化带负电荷聚合物作为 HSV-2 和 RSV 抑制剂的鉴定。
Int J Mol Sci. 2022 Aug 4;23(15):8701. doi: 10.3390/ijms23158701.
2
A physicochemical, thermodynamical, structural and computational evaluation of kynurenic acid/cyclodextrin complexes.对犬尿酸/环糊精配合物的物理化学、热力学、结构和计算评估。
Food Chem. 2021 Sep 15;356:129639. doi: 10.1016/j.foodchem.2021.129639. Epub 2021 Mar 23.
3
Lifespan extension in Caenorhabditis elegans by oxyresveratrol supplementation in hyper-branched cyclodextrin-based nanosponges.
用超支化环糊精纳米海绵包载白藜芦醇延长秀丽隐杆线虫寿命。
Int J Pharm. 2020 Nov 15;589:119862. doi: 10.1016/j.ijpharm.2020.119862. Epub 2020 Sep 8.
4
Select Drug-Drug Interactions With Direct Oral Anticoagulants: JACC Review Topic of the Week.选择直接口服抗凝药物的药物-药物相互作用：JACC 每周综述专题。
J Am Coll Cardiol. 2020 Mar 24;75(11):1341-1350. doi: 10.1016/j.jacc.2019.12.068.
5
Drug Delivery Systems: Study of Inclusion Complex Formation between Methylxanthines and Cyclodextrins and Their Thermodynamic and Transport Properties.药物传递系统：甲基黄嘌呤与环糊精包合作用的形成及其热力学和传递性质的研究。
Biomolecules. 2019 May 20;9(5):196. doi: 10.3390/biom9050196.
6
Coagulation phenotype of wild-type mice on different genetic backgrounds.不同遗传背景下野生型小鼠的凝血表型。
Lab Anim. 2019 Feb;53(1):43-52. doi: 10.1177/0023677218811059. Epub 2018 Nov 12.
7
Cyclodextrins: structure, physicochemical properties and pharmaceutical applications.环糊精：结构、物理化学性质及药物应用。
Int J Pharm. 2018 Jan 15;535(1-2):272-284. doi: 10.1016/j.ijpharm.2017.11.018. Epub 2017 Nov 11.
8
Coagulation Testing in the Core Laboratory.核心实验室中的凝血检测
Lab Med. 2017 Nov 8;48(4):295-313. doi: 10.1093/labmed/lmx050.
9
Separating and Identifying the Four Stereoisomers of Methyl Jasmonate by RP-HPLC and using Cyclodextrins in a Novel Way.通过反相高效液相色谱法分离和鉴定茉莉酸甲酯的四种立体异构体，并以一种新的方式使用环糊精。
Phytochem Anal. 2017 May;28(3):151-158. doi: 10.1002/pca.2654. Epub 2016 Dec 2.
10
Global Burden of Thrombosis: Epidemiologic Aspects.全球血栓形成负担：流行病学方面。
Circ Res. 2016 Apr 29;118(9):1340-7. doi: 10.1161/CIRCRESAHA.115.306841.