Electrochemical Sensors Research Laboratory, Department of Chemistry, Faculty of Science, Shahid Rajaee Teacher Training University, Lavizan, P.O. Box 16785-163, Tehran 167881-5811, Iran.
Physiology Research Center, Iran University of Medical Sciences, P.O. Box 14665-354, Tehran 141663-4793, Iran.
Biosensors (Basel). 2022 Dec 7;12(12):1139. doi: 10.3390/bios12121139.
Epirubicin is prescribed as an essential drug for treating breast, prostate, uterine, and gastrointestinal cancers. It has many side effects, such as heart failure, mouth inflammation, abdominal pain, fever, and shortness of breath. Its measurement is necessary by straightforward and cheap methods. The application of aptamer-based electrochemical sensors is accounted as a selective option for measuring different compounds. In this work, a thiol-modified aptamer was self-assembled on the surface of the gold electrode (AuE) boosted with carbon nano-onions (CNOs), and coupled with methylene blue (MB) as an electroactive tracker to achieve a sensitive and selective aptasensor. In the absence of the epirubicin, CNOs binds to the aptamer through a π-π interaction enhancing the MB electrochemical signal. When epirubicin binds to the aptamer, the adsorption of CNOs and MB to the aptamer is not well established, so the electrochemical signal is reduced, consequently, the epirubicin value can be measured. The prepared aptasensor demonstrated an excellent sensitivity with a curve slope of 0.36 μI/nM, and 3 nM limit of detection in the linear concentration range of 1-75 nM. The prepared aptasensor was accurately capable of measuring epirubicin in blood serum samples.
表阿霉素被规定为治疗乳腺癌、前列腺癌、子宫癌和胃肠道癌的基本药物。它有许多副作用，如心力衰竭、口腔炎症、腹痛、发烧和呼吸急促。它的测量需要通过简单而廉价的方法。基于适体的电化学传感器的应用被认为是测量不同化合物的一种选择性选择。在这项工作中，一种巯基修饰的适体被自组装在金电极（AuE）表面上，该电极表面上增强了碳纳米洋葱（CNOs），并与亚甲基蓝（MB）偶联作为电活性示踪剂，以实现灵敏和选择性的适体传感器。在没有表阿霉素的情况下，CNOs 通过 π-π 相互作用与适体结合，增强了 MB 的电化学信号。当表阿霉素与适体结合时，CNOs 和 MB 对适体的吸附不能很好地建立，因此电化学信号降低，因此可以测量表阿霉素的浓度。该制备的适体传感器具有出色的灵敏度，曲线斜率为 0.36 μI/nM，在 1-75 nM 的线性浓度范围内检测限为 3 nM。该制备的适体传感器能够准确地测量血清样品中的表阿霉素。
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