Electroanalytical Chemistry Research Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran.
Electroanalytical Chemistry Research Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran.
Biosens Bioelectron. 2017 May 15;91:650-657. doi: 10.1016/j.bios.2017.01.025. Epub 2017 Jan 14.
The present work describes a label free electrochemical aptasensor for selective detection of epirubicin. In this project, 5'-thiole terminated aptamer was self-assembled on carbon screen printed electrode, which modified with electrodeposited gold nanoparticles on magnetic double-charged diazoniabicyclo [2.2.2] octane dichloride silica hybrid (FeO@SiO/DABCO) by Au-S bond. The interactions of epirubicin with aptamer on the AuNPs/FeO@SiO/DABCO/SPE have been studied by cyclic voltammetry, linear sweep voltammetry and electrochemical impedance spectroscopy. Under optimized conditions, the peak current of epirubicin increased linearly with increasing epirubicin concentration, due to the switching in the aptamer conformation and formation of aptamer- epirubicin complex instead of aptamer on the modified electrode surface. The Apt/AuNPs/FeO@SiO/DABCO/SPE is sensitive, selective and has two linear range from 0.07µM to 1.0µM and 1.0µM to 21.0µM with a detection limit of 0.04µM. The applicability of the aptasensor was successfully assessed by determination of epirubicin in a human blood serum sample.
本工作描述了一种用于选择性检测表柔比星的无标记电化学适体传感器。在本项目中，5'-硫代末端适体通过 Au-S 键自组装在碳印刷电极上，该电极修饰有电沉积在磁性双电荷二氮杂双环[2.2.2]辛烷二氯化硅混合（FeO@SiO/DABCO）上的金纳米粒子。通过循环伏安法、线性扫描伏安法和电化学阻抗谱研究了表柔比星与 AuNPs/FeO@SiO/DABCO/SPE 上适体的相互作用。在优化条件下，由于适体构象的转变和适体-表柔比星复合物的形成而不是修饰电极表面上的适体，表柔比星的峰电流随着表柔比星浓度的增加而线性增加。Apt/AuNPs/FeO@SiO/DABCO/SPE 具有灵敏度高、选择性好的特点，在 0.07µM 至 1.0µM 和 1.0µM 至 21.0µM 范围内有两个线性范围，检测限为 0.04µM。通过测定人血清样本中的表柔比星，成功评估了适体传感器的适用性。
