Protein Research Center, Shahid Beheshti University, Tehran, Iran.
Isfahan University of Medical Sciences, Isfahan, Iran.
Biotechnol Appl Biochem. 2023 Feb;70(1):175-183. doi: 10.1002/bab.2340. Epub 2022 Apr 14.
In this work, an electrochemical aptasensor was described for the determination of prostate-specific antigen (PSA). Aptamer chains were decorated on the surface of a glassy carbon electrode (GCE) via carbon quantum dots/Au nanoparticles (Au/CQD). Structural analysis that was used to characterize the prepared materials shows that Au/CQD nanoparticles synthesized in a spherical shape with an average size of 70 nm. Furthermore, the combination of Au nanoparticles with CQD resulted in formation of crystalline the structure of the Au/CQD composite. To study the electrochemical performance of the prepared aptasensor, cyclic voltammetry, square wave voltammetry, and electrochemical impedance spectroscopy were used. The results show that the aptasensor has a good selectivity to PSA over other biomaterials with the time optimized about 30 min. K [Fe(CN) ] was used as an electrochemical probe with the limit of detection about 2 fg⋅mL . To avoid the hazardous nature of K [Fe(CN) ], a label-based aptasensor was prepared using methylene blue as an electrochemical signal producer. They provide the capability of electrochemical detection in buffer phosphate solution with high sensitivity.
本工作构建了一种电化学适体传感器，用于测定前列腺特异性抗原（PSA）。通过碳量子点/金纳米粒子（Au/CQD）将适体链修饰在玻碳电极（GCE）表面。用于表征所制备材料的结构分析表明，Au/CQD 纳米粒子呈球形，平均尺寸为 70nm。此外，金纳米粒子与 CQD 的结合导致 Au/CQD 复合结构形成结晶。为了研究制备的适体传感器的电化学性能，采用循环伏安法、方波伏安法和电化学阻抗谱法进行了研究。结果表明，该适体传感器对 PSA 具有良好的选择性，对其他生物材料的选择性约为 30 分钟。K [Fe(CN) ] 被用作电化学探针，检测限约为 2fg⋅mL 。为了避免 K [Fe(CN) ] 的危害性，使用亚甲基蓝作为电化学信号产生剂制备了基于标记的适体传感器。它们在高灵敏度的缓冲磷酸盐溶液中提供了电化学检测的能力。
