Sensors Lab, Centre for Nanoscience and Nanotechnology, Jamia Millia Islamia, New Delhi 110025, India.
Quantum and Nanophotonics Research Laboratory, Centre for Nanoscience and Nanotechnology, Jamia Millia Islamia, New Delhi 110025, India.
Biosensors (Basel). 2022 Dec 7;12(12):1140. doi: 10.3390/bios12121140.
Transition metal oxide (TMO)-based nanomaterials are effectively utilized to fabricate clinically useful ultra-sensitive sensors. Different nanostructured nanomaterials of TMO have attracted a lot of interest from researchers for diverse applications. Herein, we utilized a hydrothermal method to develop porous nanosheets of cobalt oxide. This synthesis method is simple and low temperature-based. The morphology of the porous nanosheets like cobalt oxide was investigated in detail using FESEM and TEM. The morphological investigation confirmed the successful formation of the porous nanosheet-like nanostructure. The crystal characteristic of porous cobalt oxide nanosheets was evaluated by XRD analysis, which confirmed the crystallinity of as-synthesized cobalt oxide nanosheets. The uric acid sensor fabrication involves the fixing of porous cobalt oxide nanosheets onto the GCE (glassy carbon electrode). The non-enzymatic electrochemical sensing was measured using CV and DPV analysis. The application of DPV technique during electrochemical testing for uric acid resulted in ultra-high sensitivity (3566.5 µAmMcm), which is ~7.58 times better than CV-based sensitivity (470.4 µAmMcm). Additionally, uric acid sensors were tested for their selectivity and storage ability. The applicability of the uric acid sensors was tested in the serum sample through standard addition and recovery of known uric acid concentration. This ultrasensitive nature of porous cobalt oxide nanosheets could be utilized to realize the sensing of other biomolecules.
过渡金属氧化物（TMO）基纳米材料被有效地用于制造临床上有用的超灵敏传感器。不同结构的 TMO 纳米材料因其多样化的应用而引起了研究人员的广泛关注。在此，我们采用水热法制备了多孔氧化钴纳米片。这种合成方法简单且基于低温。使用 FESEM 和 TEM 详细研究了多孔纳米片状氧化钴的形貌。形态研究证实了多孔纳米片状结构的成功形成。通过 XRD 分析评估了多孔氧化钴纳米片的晶体特性，该分析证实了合成的氧化钴纳米片的结晶度。尿酸传感器的制备涉及将多孔氧化钴纳米片固定在 GCE（玻碳电极）上。使用 CV 和 DPV 分析测量非酶电化学传感。在电化学测试中应用 DPV 技术检测尿酸可获得超高灵敏度（3566.5 µAmMcm），比基于 CV 的灵敏度（470.4 µAmMcm）高约 7.58 倍。此外，还测试了尿酸传感器的选择性和存储能力。通过标准添加和已知尿酸浓度的回收，在血清样本中测试了尿酸传感器的适用性。多孔氧化钴纳米片的这种超灵敏特性可用于实现对其他生物分子的传感。
