Noppawan Pakin, Jakmunee Jaroon, Hunt Andrew J, Supanchaiyamat Nontipa, Sangon Suwiwat, Lerdsri Jamras, Kruatian Thidarat, Upan Jantima
Department of Chemistry, Faculty of Science, Mahasarakham University, Maha Sarakham, 44150, Thailand.
Research Laboratory for Analytical Instrument and Electrochemistry Innovation, Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand.
Sci Rep. 2025 May 26;15(1):18380. doi: 10.1038/s41598-025-03405-w.
A novel and facile electrochemical sensor for the quantification of uric acid has been fabricated through the strategic modification of a screen-printed carbon electrode (SPCE) using mesoporous carbon-zinc oxide (MC-ZnO) synthesized from rice straw waste. The MC-ZnO materials, generated via a controlled pyrolysis process, exhibit homogeneous dispersion and a substantial electroactive surface area of 0.1286 cm. The electrochemical oxidation of uric acid exhibits a distinct peak at 0.18 V in differential pulse voltammetry (DPV), indicative of efficient charge transfer kinetics. A linear range spanning 20 to 225 µM was obtained with a limit of detection (LOD) of 3.76 µM, signifying exceptional analytical sensitivity. Furthermore, the sensor demonstrates robust selectivity toward uric acid in the presence of typical interferents, underscoring its applicability for precise uric acid determination in complex biological matrices. The sensor's analytical performance was validated by quantifying uric acid in spiked urine samples, yielding recovery rates between 97.9% and 114.8% and relative standard deviations (RSD) below 4.92%, affirming its accuracy and precision. This platform heralds a promising avenue for clinical diagnostics, leveraging sustainable materials for uric acid detection.
一种用于定量测定尿酸的新型便捷电化学传感器已通过使用由稻草废料合成的介孔碳-氧化锌（MC-ZnO）对丝网印刷碳电极（SPCE）进行策略性修饰而制备。通过可控热解过程生成的MC-ZnO材料表现出均匀分散性，且具有0.1286平方厘米的较大电活性表面积。在差分脉冲伏安法（DPV）中，尿酸的电化学氧化在0.18 V处呈现出一个明显的峰，这表明电荷转移动力学高效。获得了20至225 μM的线性范围，检测限（LOD）为3.76 μM，这意味着具有出色的分析灵敏度。此外，该传感器在存在典型干扰物的情况下对尿酸表现出强大的选择性，突出了其在复杂生物基质中精确测定尿酸的适用性。通过对加标尿液样本中的尿酸进行定量，验证了该传感器的分析性能，回收率在97.9%至114.8%之间，相对标准偏差（RSD）低于4.92%，证实了其准确性和精密度。该平台利用可持续材料进行尿酸检测，为临床诊断开辟了一条充满希望的途径。
