一种用于实现微流控生物分析自动化操作的紧凑型控制系统。

A Compact Control System to Enable Automated Operation of Microfluidic Bioanalytical Assays.

机构信息

Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN 55905, USA.

Department of Biology, St. Olaf College, Northfield, MN 55057, USA.

出版信息

Biosensors (Basel). 2022 Dec 13;12(12):1160. doi: 10.3390/bios12121160.

DOI:10.3390/bios12121160

PMID:36551127

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9775492/

Abstract

We describe a control system for operating valve-enabled microfluidic devices and leverage this control system to carry out a complex workflow of plasma separation from 8 μL of whole blood followed by on-chip mixing of plasma with assay reagents for biomarker detection. The control system incorporates pumps, digital pressure sensors, a microcontroller, solenoid valves and off-the-shelf components to deliver high and low air pressure in the desired temporal sequence to meter fluid flow and actuate microvalves. Importantly, our control system is portable, which is suitable for operating the microvalve-enabled microfluidic devices in the point-of-care setting.

摘要

我们描述了一种用于操作带阀微流控设备的控制系统，并利用该控制系统来执行从 8 μL 全血中分离血浆，然后在芯片上与检测试剂混合以用于生物标志物检测的复杂工作流程。该控制系统集成了泵、数字压力传感器、微控制器、电磁阀和现成组件，以按所需时间顺序提供高、低气压，从而计量流体流量并驱动微阀。重要的是，我们的控制系统是便携式的，非常适合在即时护理环境中操作带微阀的微流控设备。