Microfluidics Laboratory, Department of Mechanical, Industrial, and Systems Engineering, University of Rhode Island, 2 East Alumni Avenue, Kingston, RI 02881, USA.
Biosensors (Basel). 2023 Feb 23;13(3):310. doi: 10.3390/bios13030310.
In this paper, we present a novel and cost-effective lab-on-paper microfluidics platform for performing ELISA autonomously, with no user intervention beyond adding the sample. The platform utilizes two Bi-Material Cantilever Valves placed in a specially designed housing. The integration of these valves in a specific channel network forms a complete fluidic logic circuit for performing ELISA on paper. The housing also incorporates an innovative reagent storage and release mechanism that minimizes variability in the volume of reagents released into the reagent pads. The platform design was optimized to minimize variance in the time of fluid wicking from the reagent pad, using a randomized design of experiment. The platform adheres to the World Health Organization's ASSURED principles. The optimized design was used to conduct an ELISA for detecting rabbit immunoglobulin G (IgG) in a buffer, with a limit of detection of 2.27 ng/mL and a limit of quantification of 8.33 ng/mL. This represents a 58% improvement over previous ELISA methods for detecting rabbit IgG in buffer using portable microfluidic technology.
本文提出了一种新颖且经济高效的纸上实验室微流控平台，可在无需用户干预的情况下（除了添加样本之外）自主进行 ELISA 实验。该平台利用两个双材料悬臂阀放置在特制的外壳中。这些阀在特定的通道网络中的集成形成了用于在纸上进行 ELISA 的完整流体逻辑电路。该外壳还集成了一种创新的试剂储存和释放机制，可最大程度地减少释放到试剂垫中的试剂体积的变化。该平台设计通过实验设计的随机化来优化最小化从试剂垫中吸取流体的时间变化的方差。该平台符合世界卫生组织的 ASSURED 原则。使用优化的设计在缓冲液中进行了检测兔免疫球蛋白 G（IgG）的 ELISA 实验，检测限为 2.27ng/mL，定量限为 8.33ng/mL。与使用便携式微流控技术在缓冲液中检测兔 IgG 的先前 ELISA 方法相比，这提高了 58%。
