Löscher W, Göbel W
Epilepsia. 1978 Oct;19(5):463-73. doi: 10.1111/j.1528-1157.1978.tb05173.x.
A quantitative gas-liquid chromatographic procedure is described for the consecutive determination of phenytoin, phenobarbital, primidone, phenylethylmalondiamide, carbamazepine, trimethadione, dimethadione, ethosuximide and valproate from a single serum specimen of 1.2 ml. After extraction from serum by two different procedures, the anticonvulsants are chromatographed without further purification on a 3% OV 17 column either with or without derivative formation by means of "on-column" methylation. Multiple internal standards are employed in order to enhance the reproducibility of drug-concentration measurement.
描述了一种定量气-液色谱法，用于从1.2毫升单一血清样本中连续测定苯妥英、苯巴比妥、扑米酮、苯乙基丙二酰胺、卡马西平、三甲双酮、二甲双酮、乙琥胺和丙戊酸盐。通过两种不同程序从血清中提取后，抗惊厥药无需进一步纯化，在3% OV 17柱上进行色谱分析，可通过“柱上”甲基化形成或不形成衍生物。采用多个内标以提高药物浓度测量的重现性。
