Külpmann W R
J Clin Chem Clin Biochem. 1980 Apr;18(4):227-32.
A gas-chromatographic method for the determination of the antiepileptic drugs carbamazepine, phenobarbital, phenytoin and primidone in the same extract of serum is presented. Saturated ammonium sulfate solution is added to 1 ml serum, followed by extraction with chloroform. The organic phase is separated and evaporated. The residue is dissolved in 100 microliter ethylacetate/acetic acid (100 ml + 1 ml) for gas-chromatography. The gas-chromatographic determination is carried out under isothermal conditions without derivatisation, using SP 2250 DA as stationary phase for the determination of phenobarbital and phenytoin, and Dexsil 300 for the determination of carbamazepine and primidone. The coefficient of variation for the precision from day to day ranges from 4.3 to 7.5%, the recovery from 93.5 to 111%. The specificity was proven by comparison with the relative retention times of about 100 drugs. The method is compared with the corresponding EMIT-tests.
介绍了一种气相色谱法，用于在同一血清提取物中测定抗癫痫药物卡马西平、苯巴比妥、苯妥英和扑米酮。向1ml血清中加入饱和硫酸铵溶液，然后用氯仿萃取。分离有机相并蒸发。残渣溶于100微升乙酸乙酯/乙酸（100ml + 1ml）中用于气相色谱分析。气相色谱测定在等温条件下进行，无需衍生化，使用SP 2250 DA作为固定相测定苯巴比妥和苯妥英，使用Dexsil 300测定卡马西平和扑米酮。每日精密度的变异系数在4.3%至7.5%之间，回收率在93.5%至111%之间。通过与约100种药物的相对保留时间比较证明了特异性。该方法与相应的EMIT试验进行了比较。
