Arai Takaomi, Kimura Shogo
Environmental and Life Sciences Programme, Faculty of Science, Universiti Brunei Darussalam, Gadong BE 1410, Brunei.
Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa 277-8564, Japan.
Biology (Basel). 2022 Nov 29;11(12):1733. doi: 10.3390/biology11121733.
To secure traceability along supply chains of foodstuffs, the spatiotemporal variability of trace elements' fingerprints (TEF) in fish otoliths provides a powerful tool to determine and discriminate the origin. Spatiotemporal variability of TEF was examined in a commercially important seafood, Japanese eel (), by means of laser ablation-inductively coupled plasma mass spectrometry (LA-ICPMS). Six elemental ratios (Na:Ca, Mg:Ca, P:Ca, K:Ca, Sr:Ca, and Ba:Ca) were determined in the otoliths of specimens originating from four aquaculture farms to examine the spatial variability and from one wild habitat over three years to examine the temporal variation. Significant temporal variation was found in Mg:Ca and Sr:Ca ratios; however, discriminant function analysis showed a lower temporal variation (50%) for the three years. Spatial variations were significant in Sr:Ca and Ba:Ca ratios, and discriminant function analysis showed high (80%) spatial variation among the four farms. Otolith TEF in the Japanese eel showed specific spatial variation among aquaculture farms but intangible temporal variation, suggesting the otolith TEF reflect each aquaculture environment. The present study shows that otolith TEF can be a reliable tool to discriminate the geographic origin of the Japanese eel.
为确保食品供应链的可追溯性，鱼类耳石中微量元素指纹图谱（TEF）的时空变异性为确定和区分其来源提供了有力工具。本研究通过激光烧蚀电感耦合等离子体质谱法（LA-ICPMS），对一种重要的商业海产品日本鳗鲡的TEF时空变异性进行了研究。测定了来自四个养殖场的样本耳石中的六个元素比率（Na:Ca、Mg:Ca、P:Ca、K:Ca、Sr:Ca和Ba:Ca），以研究空间变异性，并测定了来自一个野生栖息地的样本在三年间的元素比率，以研究时间变异性。研究发现，Mg:Ca和Sr:Ca比率存在显著的时间变化；然而，判别函数分析显示，三年间的时间变化较低（50%）。Sr:Ca和Ba:Ca比率存在显著的空间变化，判别函数分析显示，四个养殖场之间的空间变化较高（80%）。日本鳗鲡的耳石TEF在养殖场之间表现出特定的空间变化，但时间变化不明显，这表明耳石TEF反映了每个养殖环境。本研究表明，耳石TEF可以作为区分日本鳗鲡地理来源的可靠工具。
