Lin Dongzhi, Zhang Ting, Chen Yu, Fan Mengjie, Kong Rongrong, Chen Lu, Wang Yulu, Xu Jianlong, Dong Yanjun
College of Life Sciences, Shanghai Normal University, Shanghai 200234, China.
The Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, 12 South Zhong-Guan Cun Street, Beijing 100081, China.
Biology (Basel). 2022 Nov 29;11(12):1738. doi: 10.3390/biology11121738.
The multi-domain GTPase (MnmE) is conservative from bacteria to human and participates in tRNA modified synthesis. However, our understanding of how the MnmE is involved in plant chloroplast development is scarce, let alone in rice. A novel rice mutant, () was identified in this study, which apparently presented an albino phenotype at 20 °C but a normal green over 24 °C, coincided with chloroplast development and chlorophyll content. Map-based cloning and complementary test revealed the encoded a multi-domain GTPase localized in chloroplasts. In addition, the disturbance of suppressed the transcripts of certain chloroplast-related genes at low temperature, although the genes were recoverable to nearly normal levels at high temperature (32 °C), indicating that governs chloroplast development at low temperature. The multi-domain GTPase gene in rice is first reported in this study, which endorses the importance in exploring chloroplast development in rice.
多结构域GTP酶（MnmE）从细菌到人类都具有保守性，并参与tRNA修饰合成。然而，我们对MnmE如何参与植物叶绿体发育的了解很少，在水稻中更是如此。本研究鉴定出一个新的水稻突变体（），其在20℃时表现出白化表型，但在24℃以上时为正常绿色，这与叶绿体发育和叶绿素含量一致。图位克隆和互补试验表明，该基因编码一种定位于叶绿体的多结构域GTP酶。此外，该基因的干扰在低温下抑制了某些叶绿体相关基因的转录，尽管这些基因在高温（32℃）下可恢复到接近正常水平，表明该基因在低温下调控叶绿体发育。本研究首次报道了水稻中的多结构域GTP酶基因，这证实了其在探索水稻叶绿体发育中的重要性。
