State Key Laboratories of Agrobiotechnology, Joint International Research Laboratory of Crop Molecular Breeding, Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, Department of Vegetable Sciences, China Agricultural University, Beijing 100193, China.
Genes (Basel). 2022 Nov 25;13(12):2216. doi: 10.3390/genes13122216.
The leaf angle is an important factor determining plant shoot architecture that may boost crop yield by increasing photosynthetic efficiency and facilitating high-density planting. Auxin is an important phytohormone involved in leaf angle regulation. Here, we identified two Single-Nucleotide Polymorphisms (SNPs) in the Indoleacetic Acid (IAA) glucosyltransferase gene in 80 re-sequenced cucumber lines, of which the is the dominant allele associated with a small leaf pedicle angle (LPA), whereas is linked with a large LPA. was highly expressed in leaves and petioles. In natural cucumber populations, the expression of was negatively correlated with the LPA. The mutation of induced by the CRISPR-Cas9 system resulted in elevated free IAA levels and enlarged cell expansion on the adaxial side of the petiole base, thus producing a greater LPA. Consistently, exogenous IAA treatment led to increased LPA and cell size. Therefore, our findings suggest that functions as a negative regulator of LPA development via auxin-mediated cell expansion in cucumber, providing a valuable strategy for cucumber breeding with small LPAs.
叶角是决定植物茎构型的一个重要因素，它可以通过提高光合作用效率和促进高密度种植来提高作物产量。生长素是参与叶角调节的一种重要植物激素。在这里，我们在 80 个重测序的黄瓜品系中鉴定了吲哚乙酸（IAA）葡萄糖基转移酶基因中的两个单核苷酸多态性（SNP），其中 是与小叶柄角度（LPA）相关的显性等位基因，而 与大 LPA 相关。 在叶片和叶柄中高度表达。在自然黄瓜群体中， 的表达与 LPA 呈负相关。CRISPR-Cas9 系统诱导的 突变导致游离 IAA 水平升高，叶柄基部的腹侧细胞扩张增大，从而产生更大的 LPA。同样，外源 IAA 处理导致 LPA 和细胞大小增加。因此，我们的研究结果表明， 在黄瓜中通过生长素介导的细胞扩张作为 LPA 发育的负调节剂起作用，为培育具有小 LPA 的黄瓜提供了有价值的策略。
