Department of Agricultural Sciences, University of Napoli Federico II, 80055 Portici, Italy.
Department of Biology, University of Naples Federico II, 80126 Napoli, Italy.
Genes (Basel). 2022 Dec 5;13(12):2293. doi: 10.3390/genes13122293.
Plant transcription factors are involved in different developmental pathways. NAC transcription factors (No Apical Meristem, Activating Factor, Cup-shaped Cotyledon) act in various processes, e.g., plant organ formation, response to stress, and defense mechanisms. In the NAC transcription factor CUPULIFORMIS (CUP) plays a role in determining organ boundaries and lip formation, and the CUP homologs of and are involved in flower organ formation. Orchidaceae is one of the most species-rich families of angiosperms, known for its extraordinary diversification of flower morphology. We conducted a transcriptome and genome-wide analysis of orchid s, focusing on the No Apical Meristem (NAM) subfamily and genes. To check whether the homologs could be involved in the perianth formation of orchids, we performed an expression analysis on the flower organs of the orchid at different developmental stages. The expression patterns of the genes of suggest their possible role in flower development and symmetry establishment. In addition, as observed in other species, the orchid and genes seem to be regulated by the microRNA, miR164. Our results represent a preliminary study of NAC transcription factors in orchids to understand the role of these genes during orchid flower formation.
植物转录因子参与不同的发育途径。NAC 转录因子（No Apical Meristem，Activating Factor，Cup-shaped Cotyledon）在各种过程中起作用，例如植物器官的形成、对胁迫的反应和防御机制。在 NAC 转录因子 CUPULIFORMIS（CUP）中，它在决定器官边界和唇形成中起作用，而 和 的 CUP 同源物参与花器官的形成。兰科是被子植物中物种最丰富的科之一，以花形态的非凡多样化而闻名。我们对兰花进行了转录组和全基因组分析，重点是 No Apical Meristem（NAM）亚家族和 基因。为了检查 同源物是否能参与兰花的花被形成，我们在不同发育阶段对兰花 的花器官进行了表达分析。 的 基因的表达模式表明它们可能在花发育和对称性建立中起作用。此外，与其他物种一样，兰花 和 基因似乎受到 microRNA miR164 的调控。我们的结果代表了对兰花 NAC 转录因子的初步研究，以了解这些基因在兰花花形成过程中的作用。
