School of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430070, China.
Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Maize Research Institute, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
Int J Mol Sci. 2023 Nov 14;24(22):16317. doi: 10.3390/ijms242216317.
NAC transcription factors play a significant role in plant stress responses. In this study, an NAC transcription factor, with a CDS of 792 bp encoding 263 amino acids, was cloned from (L.) Gaertn. (), a minor cereal crop, which is rich in flavonoids and highly stress resistant. The transcription factor was named FtNAC10 (NCBI accession number: MK614506.1) and characterized as a member of the NAP subgroup of NAC transcriptions factors. The gene exhibited a highly conserved N-terminal, encoding about 150 amino acids, and a highly specific C-terminal. The resulting protein was revealed to be hydrophilic, with strong transcriptional activation activity. FtNAC10 expression occurred in various tissues, most noticeably in the root, and was regulated differently under various stress treatments. The over-expression of FtNAC10 in transgenic () seeds inhibited germination, and the presence of FtNAC10 enhanced root elongation under saline and drought stress. According to phylogenetic analysis and previous reports, our experiments indicate that FtNAC10 may regulate the stress response or development of through ABA-signaling pathway, although the mechanism is not yet known. This study provides a reference for further analysis of the regulatory function of FtNAC10 and the mechanism that underlies stress responses in Tartary buckwheat.
NAC 转录因子在植物应激反应中发挥着重要作用。本研究从苦荞麦（）中克隆出一个 NAC 转录因子，其 cDNA 长 792bp，编码 263 个氨基酸。苦荞麦是一种小型谷类作物，富含类黄酮，具有很强的抗逆性。该转录因子被命名为 FtNAC10（NCBI 登录号：MK614506.1），并被鉴定为 NAP 亚组 NAC 转录因子的成员。该基因表现出高度保守的 N 端，编码约 150 个氨基酸，以及高度特异的 C 端。预测的蛋白质具有亲水性，具有很强的转录激活活性。FtNAC10 在各种 组织中表达，在根中表达最明显，在各种胁迫处理下的表达水平不同。在转基因 （）种子中过表达 FtNAC10 抑制了种子的萌发，而 FtNAC10 的存在增强了在盐和干旱胁迫下的根伸长。根据系统发育分析和以往的报道，我们的实验表明 FtNAC10 可能通过 ABA 信号通路调节苦荞麦的应激反应或发育，尽管其机制尚不清楚。本研究为进一步分析 FtNAC10 的调控功能以及苦荞麦应激反应的机制提供了参考。
