正常毛长和长毛天祝白牦牛全基因组拷贝数变异分析。

Analysis of Copy Number Variation in the Whole Genome of Normal-Haired and Long-Haired Tianzhu White Yaks.

机构信息

Key Laboratory of Yak Breeding Engineering Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Science, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China.

Key Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Lanzhou 730050, China.

出版信息

Genes (Basel). 2022 Dec 18;13(12):2405. doi: 10.3390/genes13122405.

DOI:10.3390/genes13122405

PMID:36553672

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9777850/

Abstract

Long-haired individuals in the Tianzhu white yak population are a unique genetic resource, and have important landscape value. Copy number variation (CNV) is an important source of phenotypic variation in mammals. In this study, we used resequencing technology to detect the whole genome of 10 long-haired Tianzhu white yaks (LTWY) and 10 normal-haired Tianzhu white yaks (NTWY), and analyzed the differences of CNV in the genome of LTWYs and NTWYs. A total of 110268 CNVs were identified, 2006 CNVRs were defined, and the distribution map of these CNVRs on chromosomes was constructed. The comparison of LTWYs and NTWYs identified 80 differential CNVR-harbored genes, which were enriched in lipid metabolism, cell migration and other functions. Notably, some differential genes were identified as associated with hair growth and hair-follicle development (e.g., , , , , , , and ). In general, we present the first genome-wide analysis of CNV in LTWYs and NTWYs. Our results can provide new insights into the phenotypic variation of different hair lengths in Tianzhu white yaks.

摘要

天祝白牦牛群体中的长毛个体是一种独特的遗传资源，具有重要的景观价值。拷贝数变异（CNV）是哺乳动物表型变异的重要来源。本研究利用重测序技术检测了 10 头长毛天祝白牦牛（LTWY）和 10 头正常毛天祝白牦牛（NTWY）的全基因组，分析了 LTWY 和 NTWY 基因组中 CNV 的差异。共鉴定出 110268 个 CNV，定义了 2006 个 CNVR，构建了这些 CNVR 在染色体上的分布图。LTWY 和 NTWY 的比较鉴定出 80 个差异表达的 CNVR 基因，这些基因富集在脂质代谢、细胞迁移等功能中。值得注意的是，一些差异表达的基因被鉴定为与毛发生长和毛囊发育相关（例如，、、、、、、和）。总之，我们首次对 LTWY 和 NTWY 的 CNV 进行了全基因组分析。我们的研究结果可为天祝白牦牛不同长度毛发的表型变异提供新的见解。