Hachisuka Y, Ogino M, Asai H, Segawa M, Maeda I, Johgiku H, Furuya M
Department of Orthopedic Surgery, National Medical Center Hospital, Tokyo, Japan.
Nihon Seikeigeka Gakkai Zasshi. 1987 May;61(5):467-76.
Three cases of malignant giant cell tumors were studied by light microscope and electron microscope. Light microscopic observation revealed three different types of tumor cells, namely, malignant stromal cells, fibrosarcomatous cell and osteosarcomatous cells. Electron microscopic observation revealed that fibrosarcomatous cells had ultrastructure similar to that of malignant stromal cells. Both types of cells showed nuclear pseudo-inclusions (NPI), and well developed organellae related to matrix synthesis in the cytoplasm. Polysaccharide staining of the same cells revealed positive products in the cytoplasm. Osteosarcomatous cells showed irregular nuclei which contained NPI and well developed r-ER. The cells were located in the non-calcified matrix which consisted of collagen fibers. No alkaline phosphatase activity was observed in this area. These facts suggest that: (1) malignant stromal cells and fibrosarcomatous cells synthesize collagenous stroma as normal fibroblasts do, and (2) osteosarcomatous cells are devoid of the ability to promote matrix calcification.
对三例恶性巨细胞瘤进行了光镜和电镜研究。光镜观察显示有三种不同类型的肿瘤细胞，即恶性基质细胞、纤维肉瘤样细胞和成骨肉瘤细胞。电镜观察显示纤维肉瘤样细胞具有与恶性基质细胞相似的超微结构。这两种类型的细胞均显示核假包涵体（NPI），且细胞质中有与基质合成相关的发达细胞器。对相同细胞进行多糖染色显示细胞质中有阳性产物。成骨肉瘤细胞显示细胞核不规则，含有NPI和发达的粗面内质网（r-ER）。这些细胞位于由胶原纤维组成的非钙化基质中。该区域未观察到碱性磷酸酶活性。这些事实表明：（1）恶性基质细胞和纤维肉瘤样细胞与正常成纤维细胞一样合成胶原性基质，（2）成骨肉瘤细胞缺乏促进基质钙化的能力。
