School of Health and Kinesiology, University of Nebraska at Omaha, Omaha, NE 68182, USA.
School of Integrative Physiology and Athletic Training, University of Montana, Missoula, MT 59812, USA.
Int J Environ Res Public Health. 2022 Dec 19;19(24):17051. doi: 10.3390/ijerph192417051.
The purpose of the study is to determine the impact of local heating on skeletal muscle transcriptional response related to mitochondrial biogenesis and mitophagy. Twelve healthy subjects (height, 176.0 ± 11.9 cm; weight, 83.6 ± 18.3 kg; and body composition, 19.0 ± 7.7% body fat) rested in a semi-reclined position for 4 h with a heated thermal wrap (HOT) around one thigh and a wrap without temperature regulation (CON) around the other (randomized). Skin temperature, blood flow, intramuscular temperature, and a skeletal muscle biopsy from the were obtained after the 4 h intervention. Skin temperature via infrared thermometer and thermal camera was higher after HOT (37.3 ± 0.7 and 36.7 ± 1.0 °C, respectively) than CON (34.8 ± 0.7, 35.2 ± 0.8 °C, respectively, < 0.001). Intramuscular temperature was higher in HOT (36.3 ± 0.4 °C) than CON (35.2 ± 0.8 °C, < 0.001). Femoral artery blood flow was higher in HOT (304.5 ± 12.5 mL‧min) than CON (272.3 ± 14.3 mL‧min, = 0.003). Mean femoral shear rate was higher in HOT (455.8 ± 25.1 s) than CON (405.2 ± 15.8 s, = 0.019). However, there were no differences in any of the investigated genes related to mitochondrial biogenesis (, , , , , ) or mitophagy (, , , ) in response to heat ( > 0.05). These data indicate that heat application alone does not impact the transcriptional response related to mitochondrial homeostasis, suggesting that other factors, in combination with skeletal muscle temperature, are involved with previous observations of altered exercise induced gene expression with heat.
本研究旨在确定局部加热对与线粒体生物发生和线粒体自噬相关的骨骼肌转录反应的影响。12 名健康受试者（身高 176.0±11.9cm；体重 83.6±18.3kg；体成分 19.0±7.7%体脂）以半躺姿势休息 4 小时，一条大腿裹有加热热裹（HOT），另一条大腿裹有不调节温度的裹（CON）（随机）。干预 4 小时后，从股部获得皮肤温度、血流、肌内温度和骨骼肌活检。通过红外温度计和热像仪测量的皮肤温度在 HOT 后更高（分别为 37.3±0.7 和 36.7±1.0°C），而在 CON 后更低（分别为 34.8±0.7 和 35.2±0.8°C，<0.001）。HOT 中的肌内温度（36.3±0.4°C）高于 CON（35.2±0.8°C，<0.001）。HOT 中的股动脉血流（304.5±12.5mL·min）高于 CON（272.3±14.3mL·min，=0.003）。HOT 中的平均股部剪切率（455.8±25.1s）高于 CON（405.2±15.8s，=0.019）。然而，在热刺激下，与线粒体生物发生（、、、、、）或线粒体自噬（、、、）相关的任何基因的转录反应均无差异（>0.05）。这些数据表明，单独应用热不会影响与线粒体稳态相关的转录反应，这表明其他因素与骨骼肌温度一起，与先前观察到的热诱导的运动诱导基因表达改变有关。
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