Department of Chemistry, Life Science and Environmental Sustainability, University of Parma, Parco Area delle Scienze 33/A, 43124 Parma, Italy.
The Italian National Interuniversity Consortium for Environmental Sciences (CINSA), Parco Area delle Scienze 93/A, 43124 Parma, Italy.
Int J Mol Sci. 2022 Dec 13;23(24):15837. doi: 10.3390/ijms232415837.
The work focused on the analysis of two cultivars of tomato ( L.), Aragon and Gladis, under two different treatments of silicon, Low, 2 L of 0.1 mM CaSiO and High, 0.5 mM CaSiO, weekly, for 8 weeks, under stress-free conditions. We subsequently analyzed the morphology, chemical composition, and elemental distribution using synchrotron-based µ-XRF techniques, physiological, and molecular aspects of the response of the two cultivars. The scope of the study was to highlight any significant response of the plants to the Si treatments, in comparison with any response to Si of plants under stress. The results demonstrated that the response was mainly cultivar-dependent, also at the level of mitochondrial-dependent oxidative stress, and that it did not differ from the two conditions of treatments. With Si deposited mainly in the cell walls of the cells of fruits, leaves, and roots, the treatments did not elicit many significant changes from the point of view of the total elemental content, the physiological parameters that measured the oxidative stress, and the transcriptomic analyses focalized on genes related to the response to Si. We observed a priming effect of the treatment on the most responsive cultivar, Aragon, in respect to future stress, while in Gladis the Si treatment did not significantly change the measured parameters.
这项工作集中分析了两个番茄品种（L.），阿ragon 和 Gladis，在两种不同的硅处理下，低浓度为 2 L 的 0.1 mM CaSiO 和高浓度为 0.5 mM CaSiO，每周一次，共 8 周，在无胁迫条件下进行。随后，我们使用基于同步加速器的微 XRF 技术分析了形态、化学成分和元素分布，以及两个品种对硅处理的生理和分子反应。研究的范围是强调植物对硅处理的任何显著反应，与胁迫下植物对硅的任何反应进行比较。结果表明，反应主要依赖于品种，也依赖于线粒体依赖的氧化应激水平，而且与两种处理条件没有差异。硅主要沉积在果实、叶片和根系细胞的细胞壁中，处理并没有从总元素含量、测量氧化应激的生理参数以及聚焦于与硅反应相关基因的转录组分析的角度引起许多显著变化。我们观察到处理对最敏感的品种 Aragon 产生了一种启动效应，以应对未来的胁迫，而在 Gladis 中，硅处理并没有显著改变测量参数。
