Mundada Pankaj S, Barvkar Vitthal T, Umdale Suraj D, Anil Kumar S, Nikam Tukaram D, Ahire Mahendra L
Department of Botany, Savitribai Phule Pune University, Pune 411007, Maharashtra, India; Department of Biotechnology, Yashavantrao Chavan Institute of Science, Satara 415001, Maharashtra, India.
Department of Botany, Savitribai Phule Pune University, Pune 411007, Maharashtra, India.
J Hazard Mater. 2021 Feb 5;403:124078. doi: 10.1016/j.jhazmat.2020.124078. Epub 2020 Sep 22.
Finger millet, a vital nutritional cereal crop provides food security. It is a well-established fact that silicon (Si) supplementation to plants alleviates both biotic and abiotic stresses. However, precise molecular targets of Si remain elusive. The present study attempts to understand the alterations in the metabolic pathways after Si amendment under osmotic stress. The analysis of transcriptome and metabolome of finger millet seedlings treated with distilled water (DW) as control, Si (10 ppm), PEG (15%), and PEG (15%) + Si (10 ppm) suggest the molecular alterations mediated by Si for ameliorating the osmotic stress. Under osmotic stress, uptake of Si has increased mediating the diversion of an enhanced pool of acetyl CoA to lipid biosynthesis and down-regulation of TCA catabolism. The membrane lipid damage reduced significantly by Si under osmotic stress. A significant decrease in linolenic acid and an increase of jasmonic acid (JA) in PEG + Si treatment suggest the JA mediated regulation of osmotic stress. The relative expression of transcripts corroborated with the corresponding metabolites abundance levels indicating the activity of genes in assuaging the osmotic stress. This work substantiates the role of Si in osmotic stress tolerance by reprogramming of fatty acids biosynthesis in finger millet.
黍稷是一种重要的营养谷物作物，能保障粮食安全。向植物补充硅（Si）可缓解生物和非生物胁迫，这是一个已被充分证实的事实。然而，硅的确切分子靶点仍不清楚。本研究试图了解在渗透胁迫下硅处理后代谢途径的变化。以蒸馏水（DW）作为对照、硅（10 ppm）、聚乙二醇（PEG，15%）以及PEG（15%）+硅（10 ppm）处理黍稷幼苗，对其转录组和代谢组进行分析，结果表明硅介导了分子变化以缓解渗透胁迫。在渗透胁迫下，硅的吸收增加，介导了更多的乙酰辅酶A转向脂质生物合成，并下调了三羧酸循环（TCA）分解代谢。在渗透胁迫下，硅显著降低了膜脂损伤。在PEG +硅处理中，亚麻酸显著减少，茉莉酸（JA）增加，这表明茉莉酸介导了对渗透胁迫的调节。转录本的相对表达与相应代谢物丰度水平相符，表明基因在缓解渗透胁迫方面的活性。这项工作证实了硅通过重新编程黍稷中脂肪酸生物合成在渗透胁迫耐受性中的作用。
