Kim R S, LaBella F S
Department of Pharmacology and Therapeutics, University of Manitoba, Faculty of Medicine, Winnipeg, Canada.
J Lipid Res. 1987 Sep;28(9):1110-7.
Oxidation profiles of polyunsaturated fatty acids, their esters, and fatty alcohols were compared by several commonly employed analytical procedures. The extent of lipid peroxidation varied with the structure of the specific lipid class. The rate of oxidation was greater for polyenoic lipids than for dienoic counterparts. In general, the maximum diene conjugation and thiobarbituric acid reactivity occurred earlier for acids than for esters or alcohols containing identical numbers of carbon chains and double bonds. For each homologous series there were, initially, increasing levels of conjugated dienes and thiobarbituric acid-reactive products, which then diminished in association with rising levels of carbonyl and fluorescent products. At late stages of oxidation, conjugated diene or thiobarbituric acid reactivity was not indicative of total peroxidation products. Thus, supplementary measurements are required to detect secondary degradation products of polyunsaturated fatty acids.
通过几种常用的分析方法比较了多不饱和脂肪酸、其酯类和脂肪醇的氧化情况。脂质过氧化的程度因特定脂质类别的结构而异。多烯脂质的氧化速率高于二烯脂质。一般来说，对于含有相同碳链数和双键数的酸、酯或醇，酸的最大二烯共轭和硫代巴比妥酸反应性出现得更早。对于每个同系物系列，最初共轭二烯和硫代巴比妥酸反应产物的水平会升高，然后随着羰基和荧光产物水平的升高而降低。在氧化后期，共轭二烯或硫代巴比妥酸反应性并不代表总的过氧化产物。因此，需要进行补充测量来检测多不饱和脂肪酸的二级降解产物。
