Rands G F
Sir William Dunn School of Pathology, University of Oxford, UK.
J Embryol Exp Morphol. 1986 Nov;98:209-17.
The study describes an analysis of the development of mouse embryos halved at the 2-cell stage by the destruction of one blastomere, in comparison with control embryos of parallel derivation, at 2.5-13.5 days post coitum. The results showed that: (1) half embryos achieve size regulation some time between 7.5 and 10.5 days; (2) there is an indication that by 13.5 days half embryos may have again dropped back significantly in size relative to controls; (3) preregulation half embryos are slightly retarded developmentally, but this does not wholly account for their smaller size: morphogenesis is not size-dependent; (4) early postimplantation half embryos contain a significantly decreased proportion of inner cell mass derivatives and increased proportion of trophectoderm derivatives when compared with controls. A comparison is also made between the up-regulation of half embryos and the down-regulation of aggregate embryos, and it is suggested that size regulation may occur by delaying a change in the normal growth rate.
该研究描述了对在2细胞阶段通过破坏一个卵裂球而减半的小鼠胚胎发育情况的分析，与同期来源的对照胚胎相比，观察时间为交配后2.5至13.5天。结果显示：（1）减半胚胎在7.5至10.5天之间的某个时间实现了大小调节；（2）有迹象表明，到13.5天时，减半胚胎的大小相对于对照胚胎可能再次显著下降；（3）预调节的减半胚胎在发育上略有延迟，但这并不能完全解释它们较小的尺寸：形态发生不依赖于大小；（4）与对照相比，植入后早期的减半胚胎中内细胞团衍生物的比例显著降低，滋养外胚层衍生物的比例增加。还对减半胚胎的上调与聚集胚胎的下调进行了比较，并提出大小调节可能是通过延迟正常生长速率的变化而发生的。
