Niculae Andrei Marian, Dobre Maria, Herlea Vlad, Vasilescu Florina, Ceafalan Laura Cristina, Trandafir Bogdan, Milanesi Elena, Hinescu Mihail Eugen
Victor Babes National Institute of Pathology, 050096 Bucharest, Romania.
Faculty of Medicine, Carol Davila University of Medicine and Pharmacy, 050474 Bucharest, Romania.
Life (Basel). 2022 Dec 16;12(12):2127. doi: 10.3390/life12122127.
The reprogramming of lipid metabolism has been highlighted in colorectal cancer (CRC) studies, suggesting a critical role for the scavenger receptor CD36 and fatty acid synthase (FASN) in this malignancy. In this study, we analyzed the gene expression levels of CD36, FASN, the cell surface glypican 4 (GPC4), and the two transporters SLC27A3 and SLC27A4 in 39 paired tumoral and peritumoral tissues from patients with CRC compared with 18 normal colonic mucosae. Moreover, the levels of seven miRNAs targeting CD36 and most of the analyzed genes were evaluated. We found a significant impairment of the expression of all the analyzed genes except GPC4 as well as the differential expression of miR-16-5p, miR-26b-5p, miR-107, miR-195-5p, and miR-27a-3p in the colonic mucosa of CRC patients. Interestingly, CD36 and miR-27a-3p were downregulated and upregulated, respectively, in tumoral tissues compared to peritumoral and control tissues, with a significant negative correlation in the group of patients developing lymph node metastasis. Our results sustain the relationship between CRC and fatty acid metabolism and emphasize the importance of related miRNAs in developing new therapeutic strategies.
脂质代谢重编程在结直肠癌（CRC）研究中受到关注，提示清道夫受体CD36和脂肪酸合酶（FASN）在这种恶性肿瘤中起关键作用。在本研究中，我们分析了39对CRC患者肿瘤组织和瘤旁组织中CD36、FASN、细胞表面磷脂酰肌醇蛋白聚糖4（GPC4）以及两种转运蛋白SLC27A3和SLC27A4的基因表达水平，并与18例正常结肠黏膜进行比较。此外，还评估了靶向CD36和大多数分析基因的7种miRNA的水平。我们发现，除GPC4外，所有分析基因的表达均有显著受损，且CRC患者结肠黏膜中miR-16-5p、miR-26b-5p、miR-107、miR-195-5p和miR-27a-3p存在差异表达。有趣的是，与瘤旁组织和对照组织相比，肿瘤组织中CD36和miR-27a-3p分别下调和上调，在发生淋巴结转移的患者组中存在显著负相关。我们的结果支持了CRC与脂肪酸代谢之间的关系，并强调了相关miRNA在开发新治疗策略中的重要性。
