从小鼠消减cDNA文库中克隆并测序鼠源T3γ cDNA
Cloning and sequencing of murine T3 gamma cDNA from a subtractive cDNA library.
作者信息
Haser W G, Saito H, Koyama T, Tonegawa S
机构信息
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139.
出版信息
J Exp Med. 1987 Oct 1;166(4):1186-91. doi: 10.1084/jem.166.4.1186.
Abstract
The coding sequences of the murine and human T3 gamma chains are of identical length (182 amino acids) and contain a remarkable conservation of residues. The most striking observation is the high degree of homology between the murine and human cytosolic domains (89%), suggesting that the effector function of the T3 complex may be extremely similar or identical within human and murine lymphocytes. Both murine and human T lymphocytes can express two T3 gamma mRNA transcripts, suggesting that a second polyadenylation signal is present downstream. A poly(A) tail is not found in the 3' untranslated region of the murine gamma presented here, indicating that the murine clones analyzed represent mRNA generated by reading through the overlapping poly(A) signals at position 850-860 and possibly terminating at a position that would produce the 1.0 kb transcript.
摘要
小鼠和人类T3γ链的编码序列长度相同（182个氨基酸），并且包含显著保守的残基。最引人注目的观察结果是小鼠和人类胞质结构域之间的高度同源性（89%），这表明T3复合物的效应功能在人类和小鼠淋巴细胞中可能极其相似或相同。小鼠和人类T淋巴细胞都可以表达两种T3γ mRNA转录本，这表明下游存在第二个聚腺苷酸化信号。此处呈现的小鼠γ链的3'非翻译区未发现聚腺苷酸尾巴，这表明所分析的小鼠克隆代表通过读取850 - 860位重叠聚腺苷酸化信号产生的mRNA，并且可能在产生1.0 kb转录本的位置终止。
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